Objective: To identify dihydroflavonol glycoside isomers in Smilax glabra.

Method: The sample was analyzed by HPLC-MS in combination with HPLC-1H-NMR.

Result: Four dihydroflavonol glycoside isomers were identified as astilbin, neoastilbin, isoastilbin, neoisoastilbin.

Conclusion: The method is simple and rapid for the identification of dihydroflavonol glycosides in S. glabra.

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