To investigate the disease-related proteins and understand molecular mechanism of mastitis at the protein level, this project presents the protein changes in the mammary gland between healthy cows and clinical mastitic cows using two-dimensional gel electrophoresis (2-DE), after stained with colloidal Coomassie Bright Blue, six spots of differentially expressed protein were detected by PDQuest software and subjected to ion trap mass spectrometer equipped with a HPLC system, and five proteins were identified. Hemoglobin beta, kappa-casein and tryptophanyl-tRNA-synthetase (TrpRS) in healthy dairy cows, while hemoglobin beta, cytochrome C oxidase and annexin V in clinical mastitic cows were identified, they were involved in binding, transport and catalytic activity. The results may provide valuable information for the investigating of the host mammary immune system response to defense against pathogens at the protein level and potential protein targets for treatment.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11259-008-9177-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Disintegrin and Metalloproteinase 17 Disrupts Bovine Macrophage MER Proto-Oncogene Tyrosine Kinase Integrity to Impede Apoptotic Cell Clearance and Promote Inflammation in Clinical Mastitis.
J Agric Food Chem
December 2024
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan West Road, Beijing 100193, China.
In clinical mastitis of dairy cows, the abnormal accumulation of apoptotic cells (ACs) and subsequent secondary necrosis and inflammation pose significant concerns, with macrophage-mediated efferocytosis, crucial for ACs clearance, remaining unexplored in this context. In nonruminants, MER proto-oncogene tyrosine kinase (MERTK) receptors are essential for efferocytosis and A Disintegrin and Metalloproteinase 17 (ADAM17) is thought to play a role in regulating MERTK integrity. This study aimed to delineate the in situ role of efferocytosis in clinical mastitis, with a particular focus on the interaction between MERTK and ADAM17 in bovine macrophages.
View Article and Find Full Text PDFContinuous sampling of healthy and mastitic quarters of lactating cattle by ultrafiltration after intramammary ceftiofur hydrochloride administration.
J Vet Intern Med
September 2024
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.
Background: Pharmacological activity of intramammary drugs depends on adequate drug concentrations within the cistern, but sampling is often limited. Insight into the active drug concentration within the mammary cistern may assist in determining effective and appropriate therapeutic decisions for cows being treated for mastitis.
Objective: Evaluate the disposition of ceftiofur hydrochloride administered intramammary in diseased and nondiseased quarters.
Characterization and antioxidant activity of nano-formulated berberine and cyperus rotundus extracts with anti-inflammatory effects in mastitis-induced rats.
Sci Rep
August 2024
Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
Article Synopsis
- Bovine mastitis, primarily caused by pathogens like S. aureus and E. coli, poses significant challenges for the dairy industry and public health, prompting research into effective treatments.
- The study focused on creating chitosan nanoparticles loaded with berberine chloride and ethanolic extracts from Cyperus rotundus to enhance their bioavailability and stability, using various analytical techniques for characterization.
- Results showed that these nanoparticles, when administered orally to rats with mastitis, effectively reduced inflammatory markers and improved histopathological outcomes without causing harm to kidney or liver functions.
Molecular characterization of antimicrobial resistance genes of isolated from mastitic camel milk in Egypt.
Vet Res Forum
June 2024
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.
is one of the most common causes of mastitis worldwide. This study aimed to determine the prevalence and antimicrobial resistance (AMR) patterns of in mastitic milk samples collected from camel farms in Matrouh Governorate, Egypt. A total of 200 mastitic camel milk samples were evaluated for using both conventional culture-based and molecular-based methods.
View Article and Find Full Text PDFStable flies are bona fide carriers of mastitis-associated bacteria.
mSphere
July 2024
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Unlabelled: Hematophagous (stable) fly populations in dairy barns are sustained by a constant availability of cattle hosts and manure, which serve as major reservoirs of both zoonotic and opportunistic bacterial pathogens. However, the composition of the fly microbiota, the mechanisms by which flies acquire their microbiome, and the ability of potentially pathogenic bacteria to colonize and persist in fly hosts remain to be investigated. Here, we longitudinally collected fly and manure samples from two connected dairy facilities.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!