Background: Distinguishing sun-induced melanocyte atypia from residual melanoma in situ (MIS) can be challenging, particularly when working with frozen sections. Immunostains such as melanoma-associated antigen recognized by T cells (MART-1) can assist, but paraffin sections provide an optimal means of analyzing melanocyte morphology.
Objective: To verify the effectiveness of a 2-hour paraffin processing technique that uses microwave technology in the preparation of MIS sections.
Methods: Twelve MIS debulk specimens were divided into 4 pieces with each piece processed 1 of 4 ways: our 2-hour paraffin technique with hematoxylin and eosin (H&E), conventional 24-hour paraffin processing with H&E, frozen sections with H&E, and frozen sections with MART-1 immunostaining. A Mohs surgeon and a dermatopathologist compared all specimens in a blinded fashion using a 3-point ranking scale to assess ease of visualizing normal melanocytes, ease of visualizing abnormal melanocytes, and overall ability to adequately visualize epidermal and dermal structures.
Results: A nonparametric signed rank test indicated no significant differences between our microwave technique and conventional paraffin processing in all 3 criteria (p=.29, .63, .75, respectively). Our microwave technique was significantly better than frozen H&E sections for all 3 criteria (p=.046, .004, .005, respectively).
Conclusion: This rapid microwave tissue processing technique is comparable with conventional paraffin section processing.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1524-4725.2008.34316.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Single-Cell Transcriptomic Analysis Reveals Biomechanical Loading-Induced Imbalance in Bone and Fat, Leading to Ossification in Lumbar Intervertebral Disc Nucleus Pulposus Degeneration.
J Cell Physiol
January 2025
Department of Spine, Wangjing Hospital of China Academy of Chinese Medical Sciences, Beijing, China.
In this study, we explored the impact of different biomechanical loadings on lumbar spine motion segments, particularly concerning intervertebral disc degeneration (IVDD). We aimed to uncover the cellular milieu and mechanisms driving ossification in the nucleus pulposus (NP) during IVDD, a process whose underlying mechanisms have remained elusive. The study involved the examination of fresh NP tissue from the L3-S1 segment of five individuals, either with IVDD or healthy.
View Article and Find Full Text PDFThermally Conductive Shape-Stabilized Phase Change Materials Enabled by Paraffin Wax and Nanoporous Structural Expanded Graphite.
Nanomaterials (Basel)
January 2025
State Key Laboratory of Nonferrous Metals and Processes, GRIMN Group Co., Ltd., Beijing 100088, China.
Paraffin wax (PW) has significant potential for spacecraft thermal management, but low thermal conductivity and leakage issues make it no longer sufficient for the requirements of evolving spacecraft thermal control systems. Although free-state expanded graphite (EG) as a thermal conductivity enhancer can ameliorate the above problems, it remains challenging to achieve higher thermal conductivity (K) (>8 W/(m·K)) at filler contents below 10 wt.% and to mitigate the leakage problem.
View Article and Find Full Text PDFGreen dyeing of cellulose diacetate fabric with disperse dyes in a liquid paraffin medium.
Int J Biol Macromol
January 2025
Hubei Provincial Engineering Laboratory for Clean Production and High Value Utilisation of Bio-based Textile Materials, Wuhan Textile University, Wuhan 430200, China; Engineering Research Centre for Clean Production of Textile Dyeing and Printing, Ministry of Education, Wuhan Textile University, Wuhan 430200, China. Electronic address:
The conventional method of dyeing cellulose diacetate (CDA) fabric with disperse dyes consumes significant amounts of fresh water and dispersants, contributing to environmental pollution and health hazards. This study explored the use of liquid paraffin as an alternative to aqueous mediums for dyeing CDA fabric with Disperse Blue 56 dyes, eliminating the need for dispersants. An L orthogonal array was used to optimize dyeing conditions based on the color strength values.
View Article and Find Full Text PDFTET2-mediated 5-hydroxymethylcytosine of TXNIP promotes cell cycle arrest in systemic anaplastic large cell lymphoma.
Clin Epigenetics
January 2025
Department of Pathology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China.
Background: 5-Hydroxymethylcytosine (5hmC) modification represents a significant epigenetic modification within DNA, playing a pivotal role in a range of biological processes associated with various types of cancer. The role of 5hmC in systemic anaplastic large cell lymphoma (ALCL) has not been thoroughly investigated. This study aims to examine the function of 5hmC in the advancement of ALCL.
View Article and Find Full Text PDFChallenges in standardizing preimplantation kidney biopsy assessments and the potential of AI-Driven solutions.
Curr Opin Nephrol Hypertens
January 2025
Department of Microbiology, Immunology and Transplantation, KU Leuven.
Purpose Of Review: This review explores the variability in preimplantation kidney biopsy processing methods, emphasizing their impact on histological interpretation and allocation decisions driven by biopsy findings. With the increasing use of artificial intelligence (AI) in digital pathology, it is timely to evaluate whether these advancements can overcome current challenges and improve organ allocation amidst a growing organ shortage.
Recent Findings: Significant inconsistencies exist in biopsy methodologies, including core versus wedge sampling, frozen versus paraffin-embedded processing, and variability in pathologist expertise.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!