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Phylogenetic Characterization of Strains from Oysters and its Comparison with Clinical E Genotype Strain.
Foodborne Pathog Dis
January 2025
Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California (CICESE), Baja California, México.
Article Synopsis
- Researchers sequenced 21 bacterial strains from an Ensenada collection, mostly from oysters, to explore genetic differences between clinical (C-genotype) and environmental (E-genotype) strains.
- The study confirmed previous findings that identified 9 strains as E and 12 as C, using specific gene presence and virulence factors for differentiation.
- Despite the genetic distinctions between the two groups, no single element was found that could definitively indicate the potential pathogenicity of oyster isolates.
Epidemiology of Staphylococcus aureus food isolates: Comparison of conventional methods with whole genome sequencing typing methods.
Food Microbiol
January 2025
ANSES, Laboratory for Food Safety, Staphylococcus, Bacillus and Clostridium Unit (SBCL), 94706, Maisons-Alfort, France.
Article Synopsis
- The study investigates various typing methods for Staphylococcus aureus to determine the best approach for epidemiological investigations and outbreak analysis.
- Methods compared included PFGE, MLST, cgMLST, cSNP, and enterotoxin profiling on 351 S. aureus isolates, assessing their discriminatory power and reliability.
- Results showed that cgMLST and cSNP performed best in distinguishing strains and resolving population structure, while all methods effectively identified related strains in outbreak scenarios.
Early identification of the nosocomial spread of vancomycin-resistant by Fourier-transform infrared spectroscopy and performance comparison with PFGE and WGS.
Emerg Microbes Infect
December 2024
Department of Microbiology, Biomedical Diagnostic Center (CDB) and ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
Early detection of disseminating vancomycin-resistant (VREfm) in ICU wards is crucial for outbreak identification and the implementation of prompt infection control measures. Genotypic methods like pulsed-field gel electrophoresis (PFGE) and whole-genome sequencing (WGS) are costly and time-consuming, hindering rapid response due to batch dependency. Fourier-transform infrared spectroscopy (FT-IR) offers the potential for real-time outbreak detection and reliable strain typing.
View Article and Find Full Text PDFApplication of pulsed-field gel electrophoresis for molecular identification of pathogenic species in Iran: a rapid and reliable method.
Iran J Microbiol
June 2024
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Background And Objectives: Leptospirosis is a zoonotic disease caused by pathogenic serovars. The genus cannot differentiated by conventional techniques. However, identity determination of pathogenic serovar is precious of public health problems and epidemiological studies.
View Article and Find Full Text PDFVirulence genes and pulsed-field gel electrophoresis profiles of Shiga toxin-producing isolated from different food samples and patients with acute diarrhea.
Iran J Microbiol
June 2024
Department of Food Science and Technology, School of Nutrition, Isfahan University of Medical Sciences, Isfahan, Iran.
Background And Objectives: O157: H7 is one of the most important causes of hemorrhagic colitis, and hemolytic uremic syndrome. The present study aimed to isolate O157: H7 from foods and patients with hemorrhagic colitis, and identify Shiga toxin genes, phylogenetic comparison, and antibiotic resistance of the isolates.
Materials And Methods: In total 400 samples, including patients stool and food were taken in Isfahan-Iran province.
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