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Development of a photosystem II-based optical microfluidic sensor for herbicide detection. | LitMetric

AI Article Synopsis

  • Herbicides pose significant health risks to humans and animals and have contaminated soil and water due to increased agricultural use.
  • They inhibit photosystem II electron transfer, affecting photosynthesis in plants and microorganisms.
  • A new portable sensing device is proposed that detects herbicide presence by measuring hydrogen peroxide production using a chemiluminescence reaction with luminol and horseradish peroxidase.

Article Abstract

Herbicides are highly toxic for both human and animal health. The increased application of herbicides in agriculture during the last decades has resulted in the contamination of both soil and water. Herbicides, under illumination, can inhibit photosystem II electron transfer. Photosynthetic membranes isolated from higher plants and photosynthetic micro-organisms, immobilized and stabilized, can serve as a biorecognition element for a biosensor. The inhibition of photosystem II causes a reduced photoinduced production of hydrogen peroxide, which can be measured by a chemiluminescence reaction with luminol and the enzyme horseradish peroxidase. In the present work, a compact and portable sensing device that combines the production and detection of hydrogen peroxide in a single flow assay is proposed for herbicide detection.

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Source
http://dx.doi.org/10.1016/j.talanta.2008.05.060DOI Listing

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