AI Article Synopsis
- Torque Teno Virus (TTV) belongs to the Anellovirus genus and has a relatively small ssDNA genome that varies in sequence by up to 30%.
- Researchers are studying the potential pathogenicity of TTV by analyzing sequences from a patient with a type of hepatitis, identifying four mRNAs that encode seven proteins.
- The localization of these proteins shows that some reside in the nucleoli and nucleoplasm, while one protein is found in the cytoplasm, suggesting diverse roles in cellular processes.
Article Abstract
Torque Teno Virus (TTV) has been assigned to the floating genus Anellovirus. TTV ssDNA genomes have a size of 3.6 to 3.8 kb and display up to 30% nucleotide diversity. The pathogenic potential of TTV is under investigation. To address a putative link of pathogenicity with the observed sequence variations, the transcription profile of P/1C1 (genogroup 1) isolated from a patient diseased with a non A-G hepatitis was analysed. Four mRNAs were identified, which encoded the seven proteins ORF1, ORF1/1, ORF1/2, ORF2, ORF2/2, ORF3 and ORF4. Expression of the ORF1 protein and its splice variant ORF1/1 in cell culture was detected by an ORF1-specific antiserum. Analysis of N-terminal tagged P/1C1-encoded proteins revealed that ORF1, ORF1/1 and ORF1/2 were localised in the nucleoli, ORF3 and ORF4 resided in the nucleoplasm, ORF2/2 appeared either in the nucleoli or the whole nucleus while ORF2 was the only protein seen in the cytoplasm.
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| http://dx.doi.org/10.1016/j.virol.2008.08.017 | DOI Listing |
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