Background: In cervical cytology taking there has been used several devices (swab, spatula, paintbrushes, and brushes) to reduce false negative results and the necessity to exfoliate more epithelial cells from squamous columnar joint and endocervix.
Objective: To compare the quality of cervical cytology taken with Cervex-brush and with Cervex-mex, utilizing the system of Bethesda.
Material And Method: Transversal and comparative study, carried out during a period of two years, that included women from 15 to 85 years old which responded spontaneously to Papanicolaou screening as part of the permanent program of opportune diagnosis of cervical-uterine cancer. Two groups of study were formed with patients assigned in random form, in group 1 was taken cervical cytology with Cervex-brush and in group 2 with Cervex-mex; all samples were manipulated under habitual procedure and cytology were read by hospital's pathologist in blinded form.
Results: There were included 1 658 patients, sample was taken with Cervex-brush in 821, and with Cervex-mex in 837. Thirty-four percent of patients have its first cervical cytology ever. Good quality cytology samples frequency was obtained with Cervex-brush in 48.5%, and with Cervex-mex in 50.4%, with statistical difference (p < 0.05).
Conclusions: Cervex-mex design permits a better taking of endocervical cells, what reduces the number of inadequate cytologies and increases the possibility of detecting endocervical alterations.
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BMC Womens Health
January 2025
Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, UK.
Background: S. haematobium is a recognized carcinogen and is associated with squamous cell carcinoma of the bladder. Its association with high-risk(HR) human papillomavirus (HPV) persistence, cervical pre-cancer and cervical cancer incidence has not been fully explored.
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January 2025
Department of Statistical Genetics, Osaka University Graduate School of Medicine, Suita, Japan.
Aberrant immune responses to viral pathogens contribute to pathogenesis, but our understanding of pathological immune responses caused by viruses within the human virome, especially at a population scale, remains limited. We analyzed whole-genome sequencing datasets of 6,321 Japanese individuals, including patients with autoimmune diseases (psoriasis vulgaris, rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), pulmonary alveolar proteinosis (PAP) or multiple sclerosis) and coronavirus disease 2019 (COVID-19), or healthy controls. We systematically quantified two constituents of the blood DNA virome, endogenous HHV-6 (eHHV-6) and anellovirus.
View Article and Find Full Text PDFPathologica
December 2024
Department of Medicine and Health Sciences "V. Tiberio", University of Molise, Campobasso, Italy.
The search for reliable prognostic markers in oral squamous cell carcinoma (OSCC) remains a critical need. Tumor-infiltrating lymphocytes (TILs), particularly T lymphocytes, play a pivotal role in the immune response against tumors and are strongly correlated with favorable prognoses. Computational pathology has proven highly effective for histopathological image analysis, automating tasks such as cell detection, classification, and segmentation.
View Article and Find Full Text PDFPathologica
December 2024
Department of Medicine and Health Sciences "V. Tiberio", University of Molise, Campobasso, Italy.
HPV status is an important prognostic factor in oropharyngeal squamous cell carcinoma (OPSCC), with HPV-positive tumors associated with better overall survival. To determine HPV status, we rely on the immunohistochemical investigation for expression of the P16 protein, which must be associated with molecular investigation for the presence of viral DNA. We aim to define a criterion based on image analysis and machine learning to predict HPV status from hematoxylin/eosin stain.
View Article and Find Full Text PDFActa Oncol
January 2025
Department of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark; Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Background And Purpose: The similarities in biology, treatment regimens and outcome between the different human papillomavirus (HPV) associated squamous cell carcinomas (SCCs) allow for extrapolation of results generated from one SC tumor type to another. In HPV associated cancers, HPV is integrated into the tumor genome and can consequently be detected in the circulating fragments of the tumor DNA. Thus, measurement of HPV in the plasma is a surrogate for circulating tumor DNA (ctDNA) and holds promise as a clinically relevant biomarker in HPV associated cancers.
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