A continuous cell line, RBA-BC, has been derived from the buffy coat of heparinized blood of a Sprague--Dawley rat with developed RBA myeloblastosis. Separations of myeloblasts from the RBA-BC cell line was accomplished by velocity sedimentation in Verografin density gradients. The cells were monitored during long-term culture by generation time analysis. Cytochemical studies revealed the myelogenous type of RBA leukemia.
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