[Response of cryopreserved ovarian tissue after autologous implantation in mouse stimulated with gonadotrophin].

Objective: To investigate the response of cryopreserved ovarian tissue after autologous implantation in mouse stimulated with gonadotrophin.

Methods: Thirty six female mice were randomly divided into three groups, with 12 mice in each group. In group of fresh ovarian tissue, fresh ovarian tissue was implanted into kidney capsule of mice; in group of cryopreserved ovarian tissue, ovarian tissue was implanted into kidney capsule of mice after cryopreserved by vitrification for two weeks. We investigated the response of ovarian tissue two weeks later after autologous implantation stimulated with gonadotrophin. Immunohistochemistry staining method was used to observe the expression of follicle stimulating hormone receptor.

Results: Before and after stimulation with gonadotrophin, the mature follicle rate of group of fresh ovarian tissue was 2.3% and 4.2%, that of group of cryopreserved ovarian tissue was 2.3% and 4.0%, and that of group of control was 2.6% and 5.8%. Regarding the percentages of mature follicle, there were significant differences after stimulation with gonadotrophin (P < 0.05). After stimulating with gonadotrophin the percentages of mature follicle were the same in the fresh tissue group, cryopreserved tissue group and control group (P > 0.05). The integrated optical density of follicle stimulating hormone receptor of fresh ovarian tissue in antrofollicle and pre-antrofollicle were 9408 +/- 2777 and 4531 +/- 1903, that of cryopreserved ovarian tissue were 9175 +/- 3093 and 4808 +/- 1386, and that of the control ovarian tissue were 8838 +/- 2064 and 5516 +/- 1136 respectively. There was no significant difference between any two groups (P > 0.05).

Conclusion: The follicle stimulating hormone receptor is preserved by cryopreservation and transplantation, small pieces of ovarian tissue response to gonadotropin stimulation is normal.