Intervertebral disc (IVD) degeneration is a significant health concern in the USA. Tissue engineering strategies have the potential to provide a viable alternative to current treatments. Nevertheless, such approaches require a suitable biomaterial scaffold for IVD tissue regeneration. Calcium crosslinked alginate has traditionally been used for in vitro culture of nucleus pulposus (NP) cells of the IVD. However, such ionically crosslinked hydrogels lose structural integrity over time. Recently, various polymers have been modified with photopolymerizable functional groups to create covalently crosslinked hydrogels. This technology may be employed to maintain the structural and mechanical integrity of three-dimensional alginate hydrogels. In this study, photocrosslinkable alginate was synthesized and evaluated for material properties and the ability to maintain the viability of encapsulated NP cells. Photocrosslinked alginate at varying percent modifications and weight/volume percentages displayed equilibrium swelling ratios and Young's moduli of 30.52 +/- 1.782 to 43.50 +/- 1.345 and 0.5850 +/- 0.1701 to 8.824 +/- 0.6014 kPa, respectively. The viability of encapsulated NP cells was highest in hydrogels at lower percent modifications, and decreased with time in culture. Taken together, this study is the first to demonstrate that photocrosslinked alginate can be used for cellular encapsulation and synthesized with tunable material properties that may be tailored for specific applications.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jbm.a.32191 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
4D bioprinting of transformable living constructs with sustained local growth factor presentation for advanced tissue engineering applications.
Colloids Surf B Biointerfaces
December 2024
Cardiovascular Center, The First Hospital of Jilin University, Changchun, Jilin 130021, China. Electronic address:
Traditional tissue engineering strategies focus on geometrically static tissue scaffolds, lacking the dynamic capability found in native tissues. The emerging field of 4D bioprinting offers a promising method to address this challenge. However, the requirement for consistent exogenous supplementation of growth factors (GFs) during tissue maturation poses a significant obstacle for in vivo application of 4D bioprinted constructs.
View Article and Find Full Text PDFTuning Star Polymer Architecture to Tailor Secondary Structures and Mechanical Properties of Diblock Polypeptide Hydrogels for Direct Ink Writing.
Biomacromolecules
December 2024
Department of Chemistry, RCSI University of Medicine and Health Sciences, Dublin D02 YN77, Ireland.
Hydrogel three-dimensional (3D) printing has emerged as a highly valuable fabrication tool for applications ranging from electronics and biomedicine. While conventional hydrogels such as gelatin, alginate, and hyaluronic acid satisfy biocompatibility requirements, they distinctly lack reproducibility in terms of mechanical properties and 3D printability. Aiming to offer a high-performance alternative, here we present a range of amphiphilic star-shaped diblock copolypeptides of l-glutamate and l-leucine residues with different topologies.
View Article and Find Full Text PDFA quercetin nanoparticle combined with a 3D-printed decellularized extracellular matrix/ gelatin methacryloyl/sodium alginate biomimetic tumor model for the treatment of melanoma.
Int J Biol Macromol
December 2024
State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China; Zhengzhou Institute of Emerging Industrial Technology, Zhengzhou 450000, China; Cancer Hospital of Dalian University of Technology, Liaoning Cancer Hospital and Institute, Shenyang 110042, China. Electronic address:
Article Synopsis
- Traditional drug testing using 2D cell cultures fails to replicate the complex tumor microenvironment, particularly for aggressive cancers like melanoma, necessitating advanced in vitro models for research and therapy.
- This study introduces quercetin nanoparticles (QueNPs) with improved solubility and anticancer properties, developed through self-assembly with Pluronic F127 (PF127).
- A 3D printed composite scaffold combining decellularized extracellular matrix (dECM) and gelatin methacryloyl (GelMA) was created to simulate a more realistic tumor environment, demonstrating over 90% tumor cell killing by QueNPs in the 3D model by day 14, compared to 2D cultures.*
Nerve-Mimetic Adhesive Hydrogel Electroceuticals: Tailoring In Situ Physically Entangled Domains in Singular Polymers.
ACS Nano
December 2024
Department of Intelligent Precision Healthcare Convergence, Sungkyunkwan University (SKKU), Suwon 16419, Republic of Korea.
Implantable electrochemicals stand out as promising candidates for resolving peripheral nerve injuries. However, challenges persist in designing bioelectronic materials that mimic tissue due to modulus matching, conformal adhesion, and immune responses. Herein, we present a nerve-mimicking design rationale for biocompatible hydrogel-based electroceuticals with a tissue-like modulus, robust and conformal tissue adhesion, exceptional mechanical toughness, and efficient stress dissipation.
View Article and Find Full Text PDFElectrospinning of methacrylated alginate for tissue engineering applications.
RSC Adv
December 2024
Univ. Grenoble Alpes, CNRS CERMAV Grenoble 38000 France
Photo-crosslinkable methacrylated alginate derivatives (M-ALGs) were synthesized modification of sodium alginate with glycidyl methacrylate. Needle (capillary) and needleless electrospinning techniques were employed to produce their nonwoven fiber mats. Spinning parameters such as applied voltage, solution composition, and flow rate were optimized to form uniform bead-free fibers with an average diameter of about 150 nm.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!