Objective: To understand the epidemiological characteristics of enterohaemorrhagic Escherichia coli (EHEC) O157 and to determine the degree of its genetic relations.
Methods: Polymerase chain reaction (PCR) techniques and chromosomal DNA digested by restriction enzyme Xba I according to PulseNet directions by pulsed field gel electrophoresis (PFGE) method were applied to 300 E. coli O157 strains isolated from patients and animal sources from 1988 to 2005 from Henan, Jiangsu and Anhui provinces.
Results: Very high prevalence of stx2 gene in EHEC O157:H7 strains isolated from some provinces of China was found and variation existed in some strains. We got 161 PFGE patterns from 300 strains. The stx2-producing strains could be clearly separated from stx2 variation-producing strains.
Conclusion: The variability of restriction enzyme-digestion patterns of O157 genomes suggested that the presence of some genomic diversity among the strains did exist.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Analysis of ancient desiccated feces - termed paleofeces or coprolites - can unlock insights into the lives of ancient people. We collected desiccated feces from caves in the Rio Zape Valley in Mexico (725-920 CE). First, we extracted DNA with methods previously optimized for paleofeces.
View Article and Find Full Text PDFThis study, conducted between June 2022 and March 2023 in Dhaka, examined prevalence in 874 samples from vegetables, vegetable wash water, and hand swabs from vendors during summer and winter. Of the total samples, 782 (89.50%) tested positive for , with 95.
View Article and Find Full Text PDFO157 induced spontaneous bacterial peritonitis in pregnancy.
BMJ Case Rep
January 2025
Obstetrics and Gynecology, All India Institute of Medical Sciences Bhopal, Bhopal, Madhya Pradesh, India.
Antibacterial activity and multi-target mechanism of harmane against Escherichia coli O157:H7 and its application on ready-to-eat leafy greens.
Int J Food Microbiol
January 2025
School of Life Science and Technology, Shandong Second Medical University, Weifang, Shandong, China. Electronic address:
Escherichia coli O157:H7 has caused many foodborne disease outbreaks and resulted in unimaginable economic losses. With the evolution of food consumption, people prefer natural preservatives. In this study, the natural agent harmane exhibited potential activity against E.
View Article and Find Full Text PDFEnhanced Detection of Viable O157:H7 in Romaine Lettuce Wash Water Using On-Filter Propidium Monoazide-Quantitative PCR.
Microorganisms
December 2024
Joint Institute for Food Safety and Applied Nutrition, University of Maryland, College Park, MD 20742, USA.
Accurate detection of viable O157:H7 in fresh produce wash water is critical for ensuring food safety and mitigating foodborne illnesses. This study evaluated an optimized on-filter propidium monoazide (PMA)-quantitative PCR (qPCR) method for detecting viable O157:H7 in romaine lettuce wash water, involving PMA pretreatment on a filter to block DNA amplification from dead cells. The method consistently detected viable cells across chemical oxygen demand (COD) levels of 1000 and 200 mg O/L, with no significant differences ( > 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!