AI Article Synopsis
- The study examines how Acinetobacter baumannii (AB) plasmids carrying three beta-lactamase genes can be transferred to E. coli through a process called conjugation.
- Researchers isolated multi-drug resistant AB from burn wounds and successfully transferred its plasmids to E. coli, analyzing drug sensitivity and stability through PCR methods.
- The findings indicate that the drug-resistant characteristics, including the transfer of specific beta-lactamase genes (bla(TEM-1), bla(PER-1), and bla(OXA-23)), are stably passed on to future generations of E. coli, contributing to AB's multi-drug resistance.
Article Abstract
Objective: To study the transferrable character of Acinetobacter baumannii (AB) plasmids with 3 types of beta-lactamase gene.
Methods: The plasmid of multi-drug resistant AB (donor) isolated from burn wound were transferred to E. coil ATCC25922 (receptor) through conjugation, and drug sensitivity was also observed. Drug-resistant gene and stability of filial generation and zygote were analyzed by PCR.
Results: The drug-resistance of donor plasmids to Sulfamethoxazole, Ampicillin, Cefalotin, Cefpodoxime, Cefuroxime, Imipenem/Cilastatin and Ampicillin/Sulbactam, and three types of beta-lactamase gene were transferred to the receptor, and were also stably transmitted for passages. The minimum inhibitor concentration of receptor to Sulfamethoxazole was > 2 mg/L after conjugation with donor, and inhibitory character could be transferred to next generation.
Conclusion: bla(TEM-1), bla(PER-1) and bla(OXA-23) genes carried in the plasmid of AB can be transferred through conjugation and stably transmitted for passages, and it is one of the molecular mechanisms for AB with multi-drug resistance after burn infections.
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