J Clin Virol
Departamento de Virología, Centro Nacional de Diagnóstico y Referencia, Ministerio de Salud, Apartado Postal 2900, Managua, Nicaragua.
Published: November 2008
Background: Numerous immunological approaches exist to diagnose dengue or detect dengue virus (DENV) infections.
Objectives: To determine the best immunological markers and specimen types for dengue diagnosis and for measuring incidence of DENV infection in community-based studies.
Study Design: In one study, acute- and convalescent-phase samples were collected from hospitalized suspected pediatric dengue cases in Managua, Nicaragua, from September 2003 to February 2004. A second study examined specimens collected in a community setting in Managua before and after the 2003-2004 dengue season to measure incidence of DENV infection. In both studies, detection of anti-DENV IgM, IgA, and IgG in serum, filter-paper blood spots, and saliva was compared to a gold standard performed on serum samples.
Results: For dengue diagnosis, the highest sensitivity and specificity was obtained by measuring IgM or IgA in serum or filter-paper blood spots; intermediate and poor results were obtained in saliva for IgM and IgA, respectively. Detection of IgG alone in serum, filter-paper blood spots, or saliva functioned best for measuring DENV infection.
Conclusions: Detection of IgM and IgA in serum and filter-paper blood spots yielded optimal results for diagnosis of dengue cases, whereas IgG was the best marker for measuring incidence of DENV infection.
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http://dx.doi.org/10.1016/j.jcv.2008.07.016 | DOI Listing |
J Hazard Mater
January 2025
Department of Chemistry, National Institute of Technology Calicut, Kerala 673601, India. Electronic address:
Hydrazine (NH) and hydrogen sulfide (HS) are environmental contaminants that adversely affect human health. Fluorescence-based detection methods for these analytes utilize their nucleophilicity and reducing ability. Therefore, fluorescent sensors capable of detecting and distinguishing hydrazine and HS are highly beneficial.
View Article and Find Full Text PDFImmun Inflamm Dis
November 2024
Department of Pediatrics, People's Hospital of Xiangxi Tujia and Miao Autonomous Prefecture, First Affliated Hospital of Jishou University, Jishou, China.
Backgrounds: Severe asthma is a significant health burden because children with severe asthma are vulnerable to medication-related side effects, life-threatening deterioration, and impaired quality of life. However, there is a lack of data to elucidate the role of inflammatory variables in asthma. This study aimed to compare the levels of inflammatory factors in serum and sputum in children with acute and stable asthma to those in healthy children and the ability to predict clinical response to azithromycin therapy.
View Article and Find Full Text PDFACS Appl Mater Interfaces
November 2024
Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, India.
Sensing caspase-3 activity is essential for understanding the role of apoptosis in cancer dynamics, controlling therapeutic strategies, and improving patient care in cancer treatment. In this study, we demonstrate a highly sensitive recombinant human caspase-3 (rhC3) detection technique in biological fluids. This technique uses a copper nanocluster stabilized with bovine serum albumin (BSA-CuNCs) as a metal-based fluorescent biosensor, conjugated with anti-human caspase-3 (ahC3).
View Article and Find Full Text PDFAnn Clin Transl Neurol
November 2024
Department of Neurology, Mayo Clinic, Rochester, Minnesota, USA.
Objective: This study aimed to evaluate the diagnostic accuracy of dried blood spot (DBS) compared with conventional serum Aquaporin-4-IgG (AQP4-IgG) testing.
Methods: Prospective multicenter diagnostic study was conducted between April 2018 and October 2023 across medical centers in the United States, Uganda, and the Republic of Guinea. Neuromyelitis optica spectrum disorder (NMOSD) patients and controls collected blood on filter paper cards along with concurrent serum samples.
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