AI Article Synopsis
- A direct sequencing method was developed to analyze variations in the human apolipoprotein E gene, which is linked to cholesterol levels.
- DNA amplification is done using polymerase chain reaction, followed by a semi-automatic sequencing process adaptable for clinical use.
- The method effectively identifies the gene's three alleles without the need for cloning, utilizing magnetic beads for DNA separation and immobilization.
Article Abstract
A direct sequencing approach has been used to analyze the polymorphism in the human apolipoprotein E gene. A method is described, in which the DNA is amplified by the polymerase chain reaction, immobilized, and sequenced by a semi-automatic procedure adaptable to clinical diagnosis. The three alleles of the apolipoprotein E gene, which differ from each other by two nucleotide substitutions and which influence serum cholesterol levels, were analyzed. The solid-phase method was able to resolve the correct nucleotide sequence in samples from both homozygous and heterozygous individuals. No cloning steps are needed and the immobilization and separation of the DNA is accomplished using magnetic beads.
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| http://dx.doi.org/10.1016/1050-3862(91)90027-o | DOI Listing |
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