Determination of trace alpha-fetoprotein variant by affinity adsorption solid substrate-room temperature phosphorimetry.

The 3.5-generation dendrimers (3.5G-D)-porphyrin (P) dual luminescent molecule (3.5G-D-P) was used to label concanavalin agglutinin (Con A); the product of the reaction is 3.5G-D-P-Con A. A new method for the determination of trace AFP-V by affinity adsorption solid substrate-room temperature phosphorimetry (AA-SS-RTP) was established, based on the room temperature phosphorescence (RTP) property of the product on polyamide membrane (PAM) substrate and the specific affinity adsorption (AA) reaction between 3.5G-D-P-Con A and alpha-fetoprotein variant (AFP-V), which caused the RTP of the system to be sharply enhanced, the DeltaIp was linearly correlated to the content of AFP-V. The sensitivity of the method was obviously high. It could accurately detect the content of AFP-V in serum. The results were tallied well with those obtained by the ELISA method.