A DNA-immobilized open tubular capillary column (DNA-immobilized OTC) system was developed for analysis and separation of target DNA oligomers. By combining the DNA-immobilized OTC column with a nano/micro-flow pump and a high-sensitivity UV detector, we succeeded in small-scale selective detection of target DNA oligomers (> 0.02 pmol). We designed a temperature-gradient strategy for the efficient separation of target DNA.

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http://dx.doi.org/10.1093/nass/nrn240DOI Listing

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