Intraspecific variation in 16S rRNA gene of Mycoplasma synoviae determined by DNA sequencing.

Comp Immunol Microbiol Infect Dis

Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, São Paulo, SP, Brazil.

Published: January 2010

AI Article Synopsis

  • Mycoplasma synoviae (MS) is a significant pathogen in poultry, leading to respiratory issues and joint inflammation, which impacts the Brazilian poultry industry economically.
  • Researchers sequenced and analyzed the 16S rRNA gene of 19 MS strains to assess genetic variability across different Brazilian poultry production areas, discovering various polymorphic patterns.
  • The study found that the number of polymorphic changes varied among strains and suggested that both spontaneous mutations over time and the movement of new strains due to management practices contribute to the observed genetic diversity.

Article Abstract

Mycoplasma synoviae (MS) is an important avian pathogen may cause both respiratory disease and joint inflammation synovitis in poultry, causing economic losses to the Brazilian poultry industry. The genotypic variation in 16S rRNA gene is unknown. Partial sequences of 16S rRNA gene of 19 strains of M. synoviae were sequenced and analyzed in order to obtain molecular characterization and evaluation of the genetic variability of strains from distinct Brazilian areas of poultry production. Different polymorphic patterns were observed. The number of polymorphic alterations in the studied strains ranged from 0 to 6. The nucleotide variations, including deletion, insertion and substitutions, ranged from 3 to 5. The genotypic diversity observed in this study may be explained by spontaneous mutations that may occur when a lineage remains in the same flock for long periods. The culling and reposition in poultry flocks may be responsible for the entry of new strains in different areas.

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Source
http://dx.doi.org/10.1016/j.cimid.2008.07.005DOI Listing

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