Forced dissociation of selectin-ligand bonds is crucial to such biological processes as leukocyte recruitment, thrombosis formation, and tumor metastasis. Although the bond rupture has been well known at high loading rate r(f) (>or=10(2) pN/s), defined as the product of spring constant k and retract velocity v, how the low r(f) (<10(2) pN/s) or the low k regulates the bond dissociation remains unclear. Here an optical trap assay was used to quantify the bond rupture at r(f)
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2586549 PMC http://dx.doi.org/10.1529/biophysj.108.137141 DOI Listing Publication Analysis
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