Purpose: To determine the role of vascular endothelial growth factor (VEGF) in embryonic eye development and lens differentiation.
Methods: Expression of components of the VEGF signaling pathway during lens development and in adults was characterized by beta-galactosidase staining of VEGF-LacZ mice, immunohistochemistry, and real-time (q) PCR. Embryonic eyes from wild-type mice and VEGF120/120 mice were analyzed by light microscopy and immunohistochemistry. VEGF function during lens development was analyzed using eye explants treated with VEGF-neutralizing antibody. Direct function of VEGF was demonstrated on the human lens epithelial cell line, HLE-B3.
Results: Embryonic lens epithelium and posterior lens fibers expressed VEGF and VEGFR2. qPCR revealed VEGF164 as the major isoform in embryonic lens. Transgenic mice expressing only VEGF120 (VEGF120/120 mice) showed major defects in eye development, including microphthalmia, failed lens differentiation, and hyperplastic hyaloid vessels. The lens displayed abnormal cell patterning and differentiation associated with altered c-Maf, Prox1, and p57 expression pattern in the anterior epithelium. The number of proliferating epithelial cells was drastically reduced in VEGF120/120 lenses. Altered MIP26 cellular localization and reduced E-cadherin expression in the lens epithelium were observed. VEGF-neutralization led to reduced fiber elongation of eye explants. Exogenous VEGF increased survival and proliferation of HLE-B3 cell in a dose-dependent manner.
Conclusions: Abnormalities in ocular development in VEGF120/120 mice suggest a role for VEGF not only in the formation of ocular vascular beds but also in the differentiation of the lens itself.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259621 | PMC |
| http://dx.doi.org/10.1167/iovs.08-2461 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Diversity and composition of ocular microbiota in contact lens wearers: Efficacy of liposomal ozonated oil.
Cont Lens Anterior Eye
January 2025
Department of Physics of Condensed Matter, Optics Area, University of Seville, Reina Mercedes S/N, 41012 Seville, Spain.
Purpose: To characterize the ocular surface microbiota in regular contact lens wearers with dry eyes and assess the effectiveness of reducing bacterial load using a liposomal ozonated oil solution.
Methods: This prospective, longitudinal, controlled study randomized subjects into two groups. Group A (45 subjects) received hydroxypropylmethylcellulose (HPMC, Artific®), while Group B (41 subjects) received ozonated sunflower seed oil with soybean phospholipids (OSSO, Ozonest®).
A portable optical detection system for rapid quantification of two rheumatoid arthritis biomarkers.
Anal Chim Acta
January 2025
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan; Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan. Electronic address:
Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease that causes joint damage and progressive destruction of adjacent cartilage and bones. Quick and accurate detection of rheumatoid factors (RF) and anti-cyclic citrullinated peptide antibodies (anti-CCP) in serum is effective in diagnosing RA and preventing its progression. However, current methods for detecting these two biomarkers are costly, time-consuming, labor-intensive, and require specialized equipment.
View Article and Find Full Text PDFBridging Them and Us Divisions: A Focus Group Study of Identities in Clinical Psychology Training.
Clin Teach
February 2025
Department of Primary Care and Mental Health, University of Liverpool, Liverpool, UK.
Background: The training of clinical psychologists is conducted by staff, trainees, service users and carers. Often those working in clinical psychology do so due to their own lived experiences. These stakeholders may require having to navigate both personal and professional identities.
View Article and Find Full Text PDFMercury tolerance and bioremediation potential of mountain soil bacteria: Insights from Darjeeling, containing elevated levels of mercury.
Sci Total Environ
January 2025
Molecular Biology and Tissue Culture Laboratory, Department of Tea Science, University of North Bengal, Siliguri, West Bengal, India. Electronic address:
More and more research is now being focused on the mercury contamination of remote mountain environments. This study aimed to explore the mountain soil of Tiger Hill, Darjeeling, through the lens of its mercury tolerant bacterial microbiome to characterize regional mercury pollution and isolate strains with mercury bioremediation potential. The soil bacteria isolated from the region displayed an extreme tolerance to mercury at previously unseen levels of up to 7 mg/mL.
View Article and Find Full Text PDFBrain tumour histopathology through the lens of deep learning: A systematic review.
Comput Biol Med
January 2025
Auckland Bioengineering Institute, The University of Auckland, New Zealand. Electronic address:
Problem: Machine learning (ML)/Deep learning (DL) techniques have been evolving to solve more complex diseases, but it has been used relatively little in Glioblastoma (GBM) histopathological studies, which could benefit greatly due to the disease's complex pathogenesis.
Aim: Conduct a systematic review to investigate how ML/DL techniques have influenced the progression of brain tumour histopathological research, particularly in GBM.
Methods: 54 eligible studies were collected from the PubMed and ScienceDirect databases, and their information about the types of brain tumour/s used, types of -omics data used with histopathological data, origins of the data, types of ML/DL and its training and evaluation methodologies, and the ML/DL task it was set to perform in the study were extracted to inform us of trends in GBM-related ML/DL-based research.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!