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Commercial Lysogeny Broth culture media and oxidative stress: a cautious tale.

Free Radic Biol Med

September 2014

Laboratoire de Chimie Bactérienne, Aix Marseille Université-CNRS, UMR 7283, Institut de Microbiologie de la Méditerranée, CNRS, 31 Chemin Joseph Aiguier, 13009 Marseille, France. Electronic address:

Lysogeny Broth (LB), most often misnamed Luria-Bertani medium, ranks among the most commonly used growth media in microbiology. Surprisingly, we observed that oxidative levels vary with the commercial origin of the LB ready to use powder. Indeed, growth on solid media of Escherichia coli and Salmonella derivatives lacking antioxidative stress defenses, such as oxyR mutant devoid of the H2O2-sensing transcriptional activator or Hpx(-) strains lacking catalases and peroxidases, exhibit different phenotypes on LB-Sigma or LB-Difco.

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