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Effects of nocodazole and latrunculin B on locomotion of amoeboid cells of Rhizochromulina sp. strain B44 (Heterokontophyta, Dictyochophyceae).
Protoplasma
December 2024
Institute of Cytology, Russian Academy of Sciences, Saint Petersburg, 194064, Russia.
Rhizochromulina is a genus of unicellular dictyochophycean algae (Heterokontophyta), comprising a single species R. marina and numerous strains. Recently, we described the first arctic rhizochromuline-Rhizochromulina sp.
View Article and Find Full Text PDFA Ralstonia solanacearum type III effector alters the actin and microtubule cytoskeleton to promote bacterial virulence in plants.
PLoS Pathog
December 2024
Department of Botany and Plant Pathology, and Center for Plant Biology, Purdue University, West Lafayette, Indiana, United States of America.
Cellular responses to biotic stress frequently involve signaling pathways that are conserved across eukaryotes. These pathways include the cytoskeleton, a proteinaceous network that senses external cues at the cell surface and signals to interior cellular components. During biotic stress, dynamic cytoskeletal rearrangements serve as a platform from which early immune-associated processes are organized and activated.
View Article and Find Full Text PDFEmployment of a Newly Defined In Vitro Fertilization Protocol to Determine the Cytoskeletal Machinery, DNA Damage, and Subsequent DNA Repair Resulting from Endocrine Disruption by Hexavalent Chromium in Rat Metaphase II Oocytes.
Curr Protoc
December 2024
Department of Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas.
These protocols describe a detailed method to determine the DNA damage and F-actin and microtubule defects of metaphase II oocytes caused by hexavalent chromium, Cr(VI), an endocrine disrupting chemical (EDC). The protocol provides systematic steps to determine protein expression encoded by pluripotency proteins such as Oct4, Nanog, and Cdx2 during early embryonic development. Occupational or environmental exposure to EDCs has significantly increased infertility in both men and women.
View Article and Find Full Text PDFThe first attempt in synthesis, identification, and evaluation of SEPT9 inhibitors on human oral squamous carcinomas.
Bioorg Chem
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School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. Electronic address:
Septin 9 (SEPT9), a GTPase, known as the fourth cytoskeleton, is widely expressed in various cells and tissues. The functions of SEPT9 are partly similar to other cytoskeletons as a structure protein. Further, SEPT9 can interact with other cytoskeletons, participating in actin dynamics and microtubule regulation.
View Article and Find Full Text PDFCellular and Nuclear Forces: An Overview.
Methods Mol Biol
December 2024
Raman Research Institute, Bangalore, Karnataka, India.
Biological cells sample their surrounding microenvironments using nanoscale force sensors on the cell surfaces. These surface-based force and stress sensors generate physical and chemical responses inside the cell. The inherently well-connected cytoskeleton and its physical contacts with the force elements on the nuclear membrane lead these physicochemical responses to cascade all the way inside the cell nucleus, physically altering the nuclear state.
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