In this study we have used a new method for human recombinant IL-1 beta (rIL-1 beta) purification and investigated its immunostimulatory biological activity. The IL-1 beta gene was cloned using a novel mRNA preparation from activated human blood monocytes. The purification protocol consists of extraction and two chromatographic steps using the new Soloza cation exchange resin. The purified protein was characterized electrophoretically, by amino acid analysis and reverse phase chromatography. The protein migrated on SDS-PAGE with a molecular weight of 18.200 but demonstrated the minor presence of aggregates (dimers and trimers). Specific activity of purified rIL-1 beta in comitogenic assay on mouse thymocytes was 10(8) U/mg protein. rIL-1 beta increased in a dose dependent manner proliferation of Con A-stimulated murine thymocytes, splenocytes, PHA-stimulated human peripheral blood lymphocytes and transformed B-cell lines. Comitogenic activity depended on the degree of lymphocyte preactivation and was similar to that of natural human IL-1 beta. rIL-1 beta enhanced IL-2 production by murine spleen cells and EL-4 cell line and IL-2 receptor expression by human peripheral blood mononuclear cells. It induced PGE2 release from human blood monocytes but had no effect on human neutrophil chemotaxis, phagocytosis and respiratory burst.
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Front Vet Sci
August 2018
Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United States.
The effects of recombinant interleukin-1β (rIL-1β) have been described for the middle carpal joint (MCJ). However, we are unaware of any studies that have described the cytological response of the tibiotarsal joint (TTJ) to rIL-1β or compared the clinical and cytological responses of the MCJ to the TTJ following the administration of intra-articular rIL-1β. Such information is critical for researchers planning to use rIL-1β to create acute synovitis models in horses.
View Article and Find Full Text PDFEffects of a single administration of the human recombinant interleukin 1beta (rIL-1beta, betaleukin) on the cytochrome P-450 dependent monooxygenase activity in rat liver and kidney were evaluated in intact rats and on the background of cytochrome P-450 inductors. It was found that betaleukin suppressed the CYP1A1/2-dependent ethoxyresorufin-o-deethylase activity in both liver and kidney, as well as the CYP2C-dependent dibenzylfluorescein-debenzylase and CYP2E1-dependent nitrophenol-hydroxylase activity in liver, but induced CYP3A-dependent N-demethylation of erythromycin in liver and kidney. Betaleukin also inhibited the beta-naphthoflavone-induced monooxygenase activity in liver, while only insignificantly acted upon the induced monooxygenases in kidney.
View Article and Find Full Text PDFJ Endocrinol
October 2002
Department of Zoology, University of Aberdeen, Aberdeen AB24 2TZ, UK.
The present study provides the first direct evidence that implicates fish cytokines as the effector molecules by which the immune system signals the neuroendocrine system and activates the hypothalamic-pituitary-interrenal stress axis. I.p.
View Article and Find Full Text PDFJ Immunol
August 2002
Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Injection of anti-type II collagen Ab and LPS induces arthritis in mice. The levels of IL-1 beta, IL-6, and chemokines (macrophage inflammatory protein (MIP)-1 alpha, MIP-2, and monocyte chemoattractant protein-1) in the hind paws increased with the onset of arthritis and correlated highly with arthritis scores. The level of TNF-alpha was also elevated, but only transiently.
View Article and Find Full Text PDFVet Immunol Immunopathol
August 2001
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen AB24 2TZ, Scotland, UK.
The predicted rainbow trout mature interleukin-1 beta (IL-1 beta) peptide has been produced as a recombinant protein in E. coli. The bioactivity of this molecule has been studied using trout head kidney cell preparations and a trout macrophage cell line (RTS11).
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!