The extent of chromosome rearrangements in Pyrococcus isolates from marine hydrothermal vents in Vulcano Island, Italy, was evaluated by high-throughput genomic methods. The results illustrate the dynamic nature of the genomes of the genus Pyrococcus and raise the possibility of a connection between rapidly changing environmental conditions and adaptive genomic properties.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570278 | PMC |
http://dx.doi.org/10.1128/AEM.01024-08 | DOI Listing |
ACS Synth Biol
December 2024
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, Hubei University, Wuhan 430062, Hubei, China.
Hand, foot, and mouth disease (HFMD), caused by enteroviruses, mostly including EV71, CVA6, CVA10, and CVA16, is an acute infectious disease commonly found in children. Due to no approved antiviral therapies and available vaccines, except for EV71, developing accurate diagnostic methods of HFMD is essential for controlling its spread and mitigating its impact on public health. Here, we create a MIRA-HEV-PAND multiple nucleic acid typing method that utilizes Ago to identify enterovirus type A pathogens (EV71, CVA6, CVA10, and CVA16) and universal type EVU.
View Article and Find Full Text PDFFront Microbiol
August 2024
Chaozhou People's Hospital, Shantou University Medical College, Chaozhou, China.
Introduction: Both the incidence and mortality rates associated with methicillin-resistant (MRSA) have progressively increased worldwide. A nucleic acid testing system was developed in response, enabling swift and precise detection of () and its MRSA infection status. This facilitates improved prevention and control of MRSA infections.
View Article and Find Full Text PDFPoult Sci
October 2024
Guangdong Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products, Hanshan Normal University, Chaozhou 521041, China; Industrial College of Biomedicine and Health Industry, Youjiang Medical University for Nationalities, Baise 533000, China. Electronic address:
Rapid and accurate detection of goose parvovirus (GPV) is crucial for controlling outbreaks and mitigating their economic impact on the poultry industry. This study introduces recombinase polymerase amplification combined with the Pyrococcus furiosus argonaute (RPA-PfAgo) system, a novel diagnostic platform designed to address the limitations of traditional GPV detection methods. Capitalizing on the rapid DNA amplification of RPA and stringent nucleic acid cleavage by the PfAgo protein, the RPA-PfAgo system offers high specificity and sensitivity in detecting GPV.
View Article and Find Full Text PDFFront Cell Infect Microbiol
August 2024
School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, Guangxi, China.
Human respiratory syncytial virus (HRSV) is the most prevalent pathogen contributing to acute respiratory tract infections (ARTI) in infants and young children and can lead to significant financial and medical costs. Here, we developed a simultaneous, dual-gene and ultrasensitive detection system for typing HRSV within 60 minutes that needs only minimum laboratory support. Briefly, multiplex integrating reverse transcription-recombinase polymerase amplification (RT-RPA) was performed with viral RNA extracted from nasopharyngeal swabs as a template for the amplification of the specific regions of subtypes A (HRSV) and B (HRSV) of HRSV.
View Article and Find Full Text PDFActa Biochim Biophys Sin (Shanghai)
May 2024
School of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224007, China.
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