[Comparative studies on contents of iridoid in processed products from Gardenia jasminoides].

Zhongguo Zhong Yao Za Zhi

Institute of Chinese Materia Madica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.

Published: May 2008

Objective: To compare the contents of iridoid in the different processed products from Gardenia jasminoides.

Method: Contents of geniposide and genipin gentiobioside in products were determined simultaneously by HPLC. A kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12:88) as the mobile phrase. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL x min(-1).

Result: The obtained linearity of the two components was good with that the relative coefficients (r) were over 0.9994 and the average recoveries were 101.8%, 99.1%, respectively.

Conclusion: The method is simple, accurate with good reproducibility. The results showed contents of two components are difference in the different processed products.

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