[Optimization of plantaricin production by Lactobacillus plantarum ZJ316].

Objective: To enhance the production of plantaricin by Lactobacillus plantarum ZJ316 isolated from infant feces.

Methods: We analyzed fermentation parameters influencing cell growth and plantaricin production with different medium composition and under different cultivation conditions.

Results: MRS (DE MAN, ROGOSA, SHARPE) medium was more suitable for producing bacteriocin than other media. The maximum plantaricin production was obtained in modified MRS medium containing 10 g/L maltose and 10 g/L glucose, 10 g/L yeast extract, 10 g/L tryptone and 2g/L tri-ammonium citrate, 1 mL/L Tween80, 6 g/L K2HPO4.3H2O, 5 g/L sodium acetate, 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. The optimal initial pH and temperature for plantaricin production were 6.5 and 30 degrees C for 24 h.

Conclusion: After optimizing, the production of plantaricin was increased 2.3-fold using the optimized medium, compared with in the basic MRS medium.