Aims: Synthetic media should be designed for the production of Metarhizium anisopliae conidia with improved virulence properties.
Methods And Results: A genetic algorithm (GA), demonstrated to be suitable for the design of media for spore mass production (Hutwimmer et al. 2008), was utilized for a multi-objective medium design to improve conidia yield and three proposed virulence properties of conidia: C : N ratio, germination speed and amount of spore-bound Pr1 protease. After five iterative optimizations, 52 media were improved over Sabouraud dextrose agar (SDA). Four media exhibited medium performances (a factor derived from the four single optimization variables) of around 0.7; cf. SDA = 0.532; media with enhanced properties were reached for each single optimization variable; Bioassays against Tenebrio larvae indicated also a slight improvement in virulence of conidia from designed media. A degenerated phenotype of the same strain did not exhibit differences in colony appearance, spore characteristics and virulence if grown on designed media.
Conclusions: The application of a problem-oriented GA is a practical and rapid method to design media for multi-objective purposes.
Significance And Impact Of The Study: The applicability of a GA for multi-objective medium design was demonstrated for the cultivation of anamorphic fungi on solid media.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1365-2672.2008.03872.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mesoporous Boron-Doped Carbon with Curved BC Active Sites for Highly Efficient HO Electrosynthesis in Neutral Media and Air-Supplied Environments.
Adv Mater
January 2025
Extreme Materials Research Center, Korea Institute of Science and Technology, 14-gil 5 Hwarang-ro, Seongbuk-gu, Seoul, 02792, Republic of Korea.
Hydrogen peroxide (HO) electrosynthesis via the 2e oxygen reduction reaction (ORR) is considered as a cost-effective and safe alternative to the energy-intensive anthraquinone process. However, in more practical environments, namely, the use of neutral media and air-fed cathode environments, slow ORR kinetics and insufficient oxygen supply pose significant challenges to efficient HO production at high current densities. In this work, mesoporous B-doped carbons with novel curved BC active sites, synthesized via a carbon dioxide (CO) reduction using a pore-former agent, to simultaneously achieve excellent 2e ORR activity and improved mass transfer properties are introduced.
View Article and Find Full Text PDFAcute Osmotic and pH Shock to Bioluminescent Bacteria Is Reversible in Terms of Luminescence Response.
Luminescence
January 2025
Department of Biomedical Science, Acharya Narendra Dev College, University of Delhi, New Delhi, India.
Bioluminescence inhibition (BLI) measurements in bioluminescent bacteria (BB) is perceived as a potential qualitative and quantitative indicator of hazardous materials. Acute but minor fluctuations in osmolarity and pH do not affect the living systems significantly. However, significant BLI is observed from marine BB due to acute osmolarity or pH changes that may affect the bioassay sensitivity.
View Article and Find Full Text PDFThe social support, healthy lifestyle, subjective well-being, and health-related quality of life among university students.
J Educ Health Promot
November 2024
School of Psychology, Northeast Normal University, Changchun, Jilin, China.
Background: The health-related quality of life and subjective well-being of young people need to be confirmed at the population level by identifying protective factors such as social support and their current healthy lifestyle. This study aims to examine a healthy lifestyle as a mediator of the associations between perceived social support to subjective well-being and health-related quality of life and also to analyze the associations between all these variables.
Methods And Materials: This study was conducted by cross-sectional survey of university students (N = 348) derived from ten cities/municipalities in East Kalimantan, Indonesia.
Factors affecting stroke prevention behavior in elderly people with high blood pressure.
J Educ Health Promot
November 2024
Doctor of Public Health Program, Faculty of Public Health, Mahasarakham University, Thailand.
Background: Preventing stroke in senior citizens with high blood pressure will reduce disability and mortality rates. The study examined the behaviors and factors influencing stroke prevention behavior in older people.
Material And Method: This cross-sectional study consisted of a sample group of 460 elderly individuals diagnosed with high blood pressure in the Mueang District, Chaiyaphum Province.
Mesenchymal stromal cells-extracellular vesicles: protein corona as a camouflage mechanism?
Extracell Vesicles Circ Nucl Acids
November 2024
Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Ospedale Galeazzi - Sant'Ambrogio, Milano 20157, Italy.
Mesenchymal stromal cells (MSCs) showed promising potential for regenerative and therapeutic applications for several pathologies and conditions. Their potential is mainly ascribed to the factors and extracellular vesicles (EVs) they release, which are now envisioned as cell-free therapeutics in cutting-edge clinical studies. A main cornerstone is the preferential uptake by target cells and tissues, in contrast to clearance by phagocytic cells or removal from circulation before reaching the final destination.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!