AI Article Synopsis
- The study analyzed the neutralizing antibody responses in human sera from individuals immune to DENV-3, focusing on samples collected from patients during the 2001-2002 Havana dengue epidemic.
- Twenty serum samples were evaluated for their effectiveness against seven different DENV-3 strains, showing stronger neutralizing activity against genotype III compared to genotype V.
- Results indicated that antibody levels varied by the timing of sample collection during the epidemic and that the virus may have evolved during this period, raising important questions for future dengue vaccines and understanding disease progression.
Article Abstract
To evaluate the neutralizing antibody activity of a human sera panel against seven strains of the homotypic virus. Sera were collected from DENV-3 immune individuals. Two DENV-3 genotypes and strains isolated at different time-points during the 2000 and 2001-2002 Havana epidemics were included. A panel of 20 late convalescent sera collected 16-18 months after acute illness from DF and DHF patients are studied. These individuals were infected during the 2001-2002 Havana DENV-3 epidemic. All but four sera collected from DF cases had a secondary DENV-1/DENV-3 infection. Sera neutralizing antibody titer against the seven DENV-3 strains were determined by plaque reduction neutralization technique. Sera samples were tested simultaneously. Studied sera showed higher levels of neutralizing antibodies to DENV-3 strains of genotype III compared to genotype V. Interesting, higher levels of neutralizing antibodies were detected to DENV-3 strain isolated at the end of the epidemic 2001-2002. An increased tendency of GMT of neutralizing antibodies according to epidemic evolution was observed for the 2001-2002 outbreak. In general, antibody levels in sera collected from DF cases were higher. Differences in the neutralization capacity of immune DENV-3 sera tested against two homologous genotypes including strains of the same genotype are demonstrated. Observed results suggest that virus changed in the course of the epidemic. The implications of this finding in terms of dengue pathogenesis and vaccine development need to be considered.
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| http://dx.doi.org/10.1002/jmv.21234 | DOI Listing |
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