We have developed a computational design strategy based on the alpha-helical coiled-coil to generate modular peptide motifs capable of assembling into metalloporphyrin arrays of varying lengths. The current study highlights the extension of a two-metalloporphyrin array to a four-metalloporphyrin array through the incorporation of a coiled-coil repeat unit. Molecular dynamics simulations demonstrate that the initial design evolves rapidly to a stable structure with a small rmsd compared to the original model. Biophysical characterization reveals elongated proteins of the desired length, correct cofactor stoichiometry, and cofactor specificity. The successful extension of the two-porphyrin array demonstrates how this methodology serves as a foundation to create linear assemblies of organized electrically and optically responsive cofactors.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3663296 | PMC |
| http://dx.doi.org/10.1021/ja800697g | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bioinspired Iron Porphyrin Covalent Organic Frameworks-Based Nanozymes Sensor Array: Machine Learning-Assisted Identification and Detection of Thiols.
ACS Appl Mater Interfaces
December 2024
Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
Given the crucial role of thiols in maintaining normal physiological functions, it is essential to establish a high-throughput and sensitive analytical method to identify and quantify various thiols accurately. Inspired by the iron porphyrin active center of natural horseradish peroxidase (HRP), we designed and synthesized two iron porphyrin covalent organic frameworks (Fe-COF-H and Fe-COF-OH) with notable peroxidase-like (POD) activity, capable of catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) into oxidized TMB with three distinct absorption peaks. Based on these, a six-channel nanozyme colorimetric sensor array was constructed, which could map the specific fingerprints of various thiols.
View Article and Find Full Text PDFRecent Advances in Bonding Regulation of Metalloporphyrin-Modified Carbon-Based Catalysts for Accelerating Energy Electrocatalytic Applications.
Small
December 2024
School of Chemistry and Materials Science, Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, Nanjing Normal University, Nanjing, 210023, P. R. China.
Article Synopsis
- Metalloporphyrins modified carbon-based materials are praised for their strong resistance, fast electron transfer rates, and excellent catalytic abilities, making them promising for energy electrocatalysis.
- Recent research has focused on using carbon substrates to support metalloporphyrins, creating diverse composite catalysts for different electrocatalytic tasks.
- The review explores how metalloporphyrins bond with carbon materials, categorizes their binding modes, and highlights the challenges and opportunities ahead for developing these advanced composite catalysts.
Boosting photothermal conversion through array aggregation of metalloporphyrins in bismuth-based coordination frameworks.
Chem Sci
September 2024
State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences Fuzhou 350002 China
Materials capable of efficiently converting near-infrared (NIR) light into heat are highly sought after in biotechnology. In this study, two new three-dimensional (3D) porphyrin-based metal-organic frameworks (MOFs) with a -net, CoTCPP-Bi/NiTCPP-Bi, were successfully synthesized. These MOFs feature bismuth carboxylate nodes interconnected by metalloporphyrinic spacers, forming one-dimensional (1D) arrays of closely spaced metalloporphyrins.
View Article and Find Full Text PDFTriple modal aptasensor arrays driven by CHA-mediated DNAzyme for signal-amplified atrazine pesticide accumulation monitoring in agricultural crops.
J Hazard Mater
September 2024
Jiangsu Key Laboratory of Pesticide Science, College of Sciences, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:
Developing sensors with high selectivity and sensitivity is of great significance for pesticide analysis in environmental assessment. Herein, a versatile three-way sensor array was designed for the detection of the pesticide atrazine, based on the integration of catalytic hairpin assembly (CHA) amplification and three-mode signal transducers. With atrazine, CHA was triggered to generate abundant G-quadruplex.
View Article and Find Full Text PDFThe clinical relevance of heme detoxification by the macrophage heme oxygenase system.
Front Immunol
April 2024
Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA, United States.
Heme degradation by the heme oxygenase (HMOX) family of enzymes is critical for maintaining homeostasis and limiting heme-induced tissue damage. Macrophages express HMOX1 and 2 and are critical sites of heme degradation in healthy and diseased states. Here we review the functions of the macrophage heme oxygenase system and its clinical relevance in discrete groups of pathologies where heme has been demonstrated to play a driving role.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!