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Screening of Taxol-producing fungi based on PCR amplification from Taxus. | LitMetric

Screening of Taxol-producing fungi based on PCR amplification from Taxus.

Biotechnol Lett

Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.

Published: December 2008

Genes coding for 10-deacetylbaccatin III-10-O-acetyl transferase and C-13 phenylpropanoid side chain-CoA acyltransferase were used as molecular markers for screening of Taxol-producing endophytic fungi. Using PCR, three out of 90 endophytic fungi, isolated from Taxus x media and Taxus yunnanensis, gave positive results. These 3 strains, when grown in 300 ml potato/dextrose liquid medium at 25 degrees C for 10 days, contained 100-160 microg Taxol/g dry wt of mycelium.

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Source
http://dx.doi.org/10.1007/s10529-008-9801-7DOI Listing

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