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Prion-impairing mutations in Hsp70 chaperone Ssa1: effects on ATPase and chaperone activities. | LitMetric

Prion-impairing mutations in Hsp70 chaperone Ssa1: effects on ATPase and chaperone activities.

Arch Biochem Biophys

Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 8, Room 407, LBG/NIDDK, Bethesda, MD 20892-0851, USA.

Published: October 2008

AI Article Synopsis

Article Abstract

We previously described many Hsp70 Ssa1p mutants that impair [PSI(+)] prion propagation in yeast without affecting cell growth. To determine how the mutations alter Hsp70 we analyzed biochemically the substrate-binding domain (SBD) mutant L483W and the nucleotide-binding domain (NBD) mutants A17V and R34K. Ssa1(L483W) ATPase activity was elevated 10-fold and was least stimulated by substrates or Hsp40 co-chaperones. Ssa1(A17V) and Ssa1(R34K) ATPase activities were nearly wild type but both showed increased stimulation by substrates. Peptide binding and reactivation of denatured luciferase were enhanced in Ssa1(A17V) and Ssa1(R34K) but compromised in Ssa1(L483W). The nucleotide exchange factor Fes1 influenced ATPase of wild type Ssa1 and each mutant differently. Partial protease digestion uncovered similar and distinct conformational changes of the substrate-binding domain among the three mutants. Our data suggest that prion-impairing mutations of Ssa1 can increase or decrease substrate interactions, alter the Hsp70 reaction cycle at different points and impair normal NBD-SBD cooperation.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577198PMC
http://dx.doi.org/10.1016/j.abb.2008.07.023DOI Listing

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Prion-impairing mutations in Hsp70 chaperone Ssa1: effects on ATPase and chaperone activities.

Arch Biochem Biophys

October 2008

Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 8, Room 407, LBG/NIDDK, Bethesda, MD 20892-0851, USA.

We previously described many Hsp70 Ssa1p mutants that impair [PSI(+)] prion propagation in yeast without affecting cell growth. To determine how the mutations alter Hsp70 we analyzed biochemically the substrate-binding domain (SBD) mutant L483W and the nucleotide-binding domain (NBD) mutants A17V and R34K. Ssa1(L483W) ATPase activity was elevated 10-fold and was least stimulated by substrates or Hsp40 co-chaperones.

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