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| http://dx.doi.org/10.3791/121 | DOI Listing |
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Combined biolistic and cell penetrating peptide delivery for the development of scalable intradermal DNA vaccines.
J Control Release
March 2024
Regenerative Medicine & Cellular Therapies (RMCT), Biodiscovery Institute (BDI), School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK; NIHR Nottingham Biomedical Research Centre, University of Nottingham, Nottingham, UK. Electronic address:
Physical-based gene delivery via biolistic methods (such as the Helios gene gun) involve precipitation of nucleic acids onto microparticles and direct transfection through cell membranes of exposed tissue (e.g. skin) by high velocity acceleration.
View Article and Find Full Text PDFDNA Vaccines: History, Molecular Mechanisms and Future Perspectives.
J Mol Biol
December 2023
School of Biosciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK. Electronic address:
The history of DNA vaccine began as early as the 1960s with the discovery that naked DNA can transfect mammalian cells in vivo. In 1992, the evidence that such transfection could lead to the generation of antigen-specific antibody responses was obtained and supported the development of this technology as a novel vaccine platform. The technology then attracted immense interest and high hopes in vaccinology, as evidence of high immunogenicity and protection against virulent challenges accumulated from several animal models for several diseases.
View Article and Find Full Text PDFIdentifying Factors that Determine Effectiveness of Delivery Agents in Biolistic Delivery Using a Library of Amine-Containing Molecules.
ACS Appl Bio Mater
October 2022
Department of Materials Science and Engineering, Iowa State University, Ames, Iowa 50011, United States.
Biolistic transfection is a popular and versatile tool for plant transformation. A key step in the biolistic process is the binding of DNA to the heavy microprojectile using a delivery agent, usually a positively charged molecule containing amine groups. Currently, the choice of the commercial delivery agent is mostly limited to spermidine.
View Article and Find Full Text PDFLlama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders.
Methods Mol Biol
February 2022
Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, ON, Canada.
Genetic immunization is a simple, cost-effective, and powerful tool for inducing innate and adaptive immune responses to combat infectious diseases and difficult-to-treat illnesses. DNA immunization is increasingly used in the generation of monoclonal antibodies against targets for which pure proteins are unavailable or are difficult to express and purify (e.g.
View Article and Find Full Text PDFSingle Amino Acid Deletion at N-Terminus of the Target Antigen in DNA Vaccine Induces Altered CD8 T Cell Responses against Tumor Antigen.
Vaccines (Basel)
May 2021
Department of Microbiology and Immunology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Since CD8 T cells have immunological memory and can eliminate tumor or infected cells, antigen-specific CD8 T cell inducing DNA vaccines are potential next-generation vaccines. However, the relationship between single amino acid deletion of target antigens in plasmid DNA vaccines and vaccine efficacy is not completely understood. To address this knowledge disparity and improve DNA vaccine development, two constructs cytosolic form of ovalbumin, pOVAv (346 amino acids) and pOVAy (345 amino acids) were constructed and compared.
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