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The MYB-bHLH-NRAMP module modulates the cadmium sensitivity of quinoa by regulating cadmium transport and absorption.
J Hazard Mater
January 2025
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, School of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, China. Electronic address:
Cadmium (Cd) is one of the most dangerous environmental pollutants and is easily absorbed by food crops. Quinoa is a kind of coarse grain crop with rich nutrition and strong stress resistance, which is easy to accumulate Cd. The increasingly serious soil Cd pollution poses a serious threat to the food safety of quinoa.
View Article and Find Full Text PDFIdentification and Functional Analysis of the Gene Conferring Resistance to Late Blight () in Tomato.
Plants (Basel)
December 2024
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Late blight is a destructive disease affecting tomato production. The identification and characterization of resistance (R) genes are critical for the breeding of late blight-resistant cultivars. The incompletely dominant gene confers resistance against the race T of in tomatoes.
View Article and Find Full Text PDFThe Identification of Family Genes and Their Expression, Function, and Regulation in .
Plants (Basel)
December 2024
College of Agronomy and Biotechnology, Yunnan Agricultural University (YNAU), Kunming 650201, China.
Quinoa () is an Andean allotetraploid pseudocereal crop with higher protein content and balanced amino acid composition in the seeds. Ammonium (NH), a direct source of organic nitrogen assimilation, mainly transported by specific transmembrane ammonium transporters (), plays important roles in the development, yield, and quality of crops. Many and their functions have been identified in major crops; however, no systematic analyses of and their regulatory networks, which is important to increase the yield and protein accumulation in the seeds of quinoa, have been performed to date.
View Article and Find Full Text PDFIdentification and characterization of interacting proteins of transcription factor DpWRI1-like related to lipid biosynthesis from microalga .
Heliyon
January 2025
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, Guangxi, 541006, China.
Our previous study found that WRINKLED1-like (DpWRI1-like) was a key regulatory factor of lipid biosynthesis in . gene and target genes of DpWRI1-like have been obtained in our previous study, but the interacting proteins of DpWRI1-like are unclear now, which has limited a deep understanding of the function of DpWRI1-like. Yeast two-hybrid was widely used to identify protein-protein interaction.
View Article and Find Full Text PDFNanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction.
Biosens Bioelectron
December 2024
Shenzhen Bay Laboratory, Shenzhen, 518132, China. Electronic address:
Here, we developed nanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction (NanaPPI). When an interaction occurs, primary antibodies for two proteins bring the proximity of secondary nanobody-fused small/large fragment to reassemble into an intact NanoLuc variant, thus transforming interaction events to luminescent signals in situ with high sensitivity. Compared to proximity ligation assay, NanaPPI has a similar signal-to-background ratio, but it is more convenient with faster procedures, easier readout and lower cost.
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