Which of the 20 different, commercially available, temperature cyclers adequately fulfill all functional requirements? How can an investigator analyze instrument function? This report describes in detail, for the first time, the characteristics of Ericomp temperature cyclers. The data show that the temperature uniformity, accuracy and maximum overshoot is +/- 0.34 degree C, less than 0.5 degree C and 0.17 degree C, respectively. Cycling temperature reproducibility is 0.1%, and cycling time reproducibility is 0.4%. These data compare very favourably to published data describing other temperature cyclers. The techniques used to obtain these data serve as a model for evaluating instruments for well-to-well uniformity, temperature accuracy and within-run reproducibility.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
A Thermal Cycler Based on Magnetic Induction Heating and Anti-Freezing Water Cooling for Rapid PCR.
Micromachines (Basel)
November 2024
State Key Laboratory of Intelligent Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Distinguished by its exceptional sensitivity and specificity, Polymerase Chain Reaction (PCR) is a pivotal technology for pathogen detection. However, traditional PCR instruments that employ thermoelectric cooling (TEC) are often constrained by cost, efficiency, and performance variability resulting from the fluctuations in ambient temperature. Here, we present a thermal cycler that utilizes electromagnetic induction heating at 50 kHz and anti-freezing water cooling with a velocity of 0.
View Article and Find Full Text PDFERA-CRISPR/Cas12a system: a rapid, highly sensitive and specific assay for .
Front Cell Infect Microbiol
September 2024
The Affiliated Nanhua Hospital, Department of Clinical Laboratory, Hengyang Medical School, University of South China, Hengyang, China.
Introduction: , the causative agent of human tuberculosis, poses a significant threat to global public health and imposes a considerable burden on the economy. However, existing laboratory diagnostic methods for are time-consuming and have limited sensitivity levels.
Methods: The CRISPR/Cas system, commonly known as the "gene scissors", demonstrates remarkable specificity and efficient signal amplification capabilities.
pH-dependent binding of ATP aptamer to the target and competition strands: Fluorescent melting curve fitting study.
Biochim Biophys Acta Gen Subj
November 2024
St. Petersburg University, 13B Universitetskaya Emb., St. Petersburg, 199034, Russia. Electronic address:
The pH varies in different tissues and organelles and also changes during some diseases. In this regard, the application of molecular switches that use a competition-based aptamer switch design in biological systems requires studying the thermodynamics of such systems at different pH values. In this work, we studied the binding of the classical ATP aptamer to ATP and competition strands under different pH and ionic conditions using fluorescent melting curve analysis.
View Article and Find Full Text PDFSingle-tube colorimetric loop-mediated isothermal amplification (LAMP) assay for high-sensitivity detection of SLCMV in cassava from southern India.
Microb Pathog
July 2024
ICAR-Central Tuber Crops Research Institute, Thiruvananthapuram, India. Electronic address:
Sri Lankan cassava mosaic virus (SLCMV) is a major cause for mosaic infections in cassava leaves, resulting in significant economic losses in southern India. SLCMV leads to growth retardation, leaf curl, and chlorosis in the host, with rapid transmission through whitefly insect vectors. Detecting SLCMV promptly is crucial, and the study introduces a novel and efficient colorimetric Loop-mediated isothermal amplification (LAMP) assay for successful detection in 60 min.
View Article and Find Full Text PDFThe Development of a Multienzyme Isothermal Rapid Amplification Assay to Visually Detect Duck Hepatitis B Virus.
Vet Sci
April 2024
College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Duck hepatitis B virus (DHBV) is widely prevalent in global ducks and has been identified in Chinese geese with a high prevalence; the available detection techniques are time-consuming and require sophisticated equipment. In this study, an assay combining multienzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) was developed for the efficient and rapid detection of DHBV. The primary reaction condition of the MIRA assay for DHBV detection was 10 min at 38 °C without a temperature cycler.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!