AI Article Synopsis
- Endomitosis is a process in megakaryocyte development where DNA replicates without cell division, crucial for their growth.
- A new mouse model was created to visualize chromosomal behavior in living megakaryocytes with a fluorescent protein, revealing important details about their chromosomal dynamics.
- The study found that high ploidy megakaryocytes showed unique chromosome alignment during late anaphase, differing from the patterns observed in low ploidy megakaryocytes, highlighting distinct regulatory mechanisms in these cells.
Article Abstract
Endomitosis in megakaryocytes (MKs) involves repeated DNA replication in the absence of cytokinesis and is a crucial part of MK development. However, chromosomal dynamics have never been observed in living MKs. We developed a new transgenic mouse model in which the expression of human histone H2B fused in-frame to green fluorescent protein is targeted to MKs. Ex vivo time-lapse microscopy analysis indicated that chromosomal condensation occurs at early mitosis in all MKs. In high ploidy MKs (>or=8N), late anaphase was marked by a ring-type alignment of chromosomes with multiple territories formed between them. By contrast, in low ploidy MKs mitotic chromosomes segregated to form two groups separated by a clear space before re-joining to one cluster. This is the first study to document chromosomal segregation patterns during endomitosis ex vivo and to indicate their potential differential regulation in low and high ploidy cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861775 | PMC |
| http://dx.doi.org/10.4161/cc.6325 | DOI Listing |
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