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[Analysis of the allergenicity and immunogenicity of Psilogramma menephron allergen]. | LitMetric

[Analysis of the allergenicity and immunogenicity of Psilogramma menephron allergen].

Nan Fang Yi Ke Da Xue Xue Bao

Respiratory Department of the Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Shannxi, Xi'an 710004, China.

Published: July 2008

AI Article Synopsis

  • The study aims to investigate the allergenicity and immunogenicity of the Psilogramma menephron allergen to aid in developing standardized allergen vaccines.
  • Researchers used SDS-PAGE to analyze extracts and tested their effects on 9 allergic patients' serum through immunoblotting.
  • The findings identified key allergenic proteins with molecular weights of 74,000 and 36,000 as well as immunogenic proteins at 79,000 and 25,000, which are crucial for diagnosing and treating Psilgramma menephron allergies.

Article Abstract

Objective: To analyze the allergenicity and immunogenicity of Psilogramma menephron allergen so as to provide the basis for preparing recombinant and standardized allergen vaccines of Psilgramma menephorn.

Methods: The extracts of Psilgramma menephorn were analyzed by SDS-PAGE, and the allergenicity and immunogenicity of the extracts were tested with 9 sera from allergic patients by means of immunoblotting.

Results: More than 20 allergen proteins were separated from the extract of Psilgramma menephorn by SDS-PAGE, with the relative molecular weight ranging from 12,000 to 128,000. The relative molecular weight of the allergenic proteins were 74,000 (88.9%), 66,000 (22.2%), 49,000 (22.2%), 36,000 (77.8%), or 25,000 (33.3%), and those of the immunogenic proteins were 79,000 (33.3%), 74,000 (66.7%), 66,000 (22.2%), 49,000 (22.2%), 36,000 (44.4%), or 25,000 (55.6%).

Conclusion: The relative molecular weight of the major allergenic proteins of Psilgramma menephorn are 74,000 and 36,000, and 74,000 and 25,000 for the major immunogenic proteins. These proteins constitute the major allergenic components for diagnosis and specific treatment of Psilgramma menephorn allergy.

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