Background: Respiratory infections are the most common infectious diseases in humans worldwide and are a leading cause of death in children less than 5 years of age.
Objectives: Identify candidate pathogens in pediatric patients with unexplained respiratory disease.
Study Design: Forty-four nasopharyngeal washes collected during the 2004-2005 winter season from pediatric patients with respiratory illnesses that tested negative for 7 common respiratory pathogens by culture and direct immunofluorescence assays were analyzed by MassTag-PCR. To distinguish human enteroviruses (HEV) and rhinoviruses (HRV), samples positive for picornaviruses were further characterized by sequence analysis.
Results: Candidate pathogens were detected by MassTag PCR in 27 of the 44 (61%) specimens that previously were rated negative. Sixteen of these 27 specimens (59%) contained picornaviruses; of these 9 (57%) contained RNA of a recently discovered clade of rhinoviruses. Bocaviruses were detected in three patients by RT-PCR.
Conclusions: Our study confirms that multiplex MassTag-PCR enhances the detection of pathogens in clinical specimens, and shows that previously unrecognized rhinoviruses, that potentially form a species HRV-C, may cause a significant amount of pediatric respiratory disease.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2603178 | PMC |
| http://dx.doi.org/10.1016/j.jcv.2008.06.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of a multiplex polymerase chain reaction for early diagnosis of ventriculostomy-related infections.
J Neurosurg
December 2015
Division of Infectious Disease, Department of Medicine, and.
Object: Diagnosis of ventriculostomy-related infections (VRIs) is challenging due to the lack of rapid, sensitive assays for pathogen detection. The authors report the development of a multiplex polymerase chain reaction (PCR) assay for differential diagnosis of common VRI pathogens.
Methods: MassTag PCR was used to develop a multiplex assay for detection of 11 VRI pathogens.
Multiplex PCR analysis of clusters of unexplained viral respiratory tract infection in Cambodia.
Virol J
December 2014
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York City, USA.
Background: Fevers of unknown origin constitute a substantial disease burden in Southeast Asia. In majority of the cases, the cause of acute febrile illness is not identified.
Methods: We used MassTag PCR, a multiplex assay platform, to test for the presence of 15 viral respiratory agents from 85 patients with unexplained respiratory illness representing six disease clusters that occurred in Cambodia between 2009 and 2012.
No agent is implicated in most central nervous system (CNS) infections. To investigate cerebrospinal fluid samples from patients with CNS infections of unknown cause in 1 hospital in Taiwan, we used a staged molecular approach, incorporating techniques including multiplex MassTag PCR, 16S rRNA PCR, DNA microarray, and high-throughput pyrosequencing. We determined the infectious agent for 31 (24%) of 131 previously negative samples.
View Article and Find Full Text PDFEvaluation of a pilot respiratory virus surveillance system linking electronic health record and diagnostic data.
J Public Health Manag Pract
February 2014
Division of Epidemiology, New York City Department of Health and Mental Hygiene, New York, NY, USA.
Context: During the onset of 2009 pandemic influenza A (H1N1) (pH1N1), the New York City Department of Health and Mental Hygiene implemented a pilot respiratory virus surveillance system.
Objectives: We evaluated the performance of this pilot system, which linked electronic health record (EHR) clinical, epidemiologic, and diagnostic data to monitor influenza-like illness (ILI) in the community.
Design: Surveillance was conducted at 9 community health centers with EHRs.
Comparison of real-time PCR and MassTag PCR for the multiplex detection of highly pathogenic agents.
Mol Cell Probes
October 2012
Centre for Biological Security, Robert Koch Institute, Berlin, Germany.
Multiplex PCR assays are a cost- as well as labour-effective way to analyse one sample for several pathogens simultaneously. Besides the mutual competition of the individual PCR reactions included in a multiplex PCR assay, their specific read-out displays a limiting factor for the total number of PCR reactions that can be multiplexed. In this study, two PCR systems with different read-out approaches are compared, using a pentaplex PCR assay for the detection of highly pathogenic agents.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!