AI Article Synopsis
- This review discusses a new mixed-mode HPLC method called hydrophilic interaction/cation-exchange chromatography (HILIC/CEX), which effectively separates and analyzes peptides and proteins.
- It combines two different separation methods: one based on the polarity of peptides (hydrophilicity/hydrophobicity) and the other based on their net charge.
- The approach has shown strong results with various types of peptides, including cyclic and modified histone proteins, and serves as a valuable complement to reverse-phase chromatography (RPC) in analytical applications.
Article Abstract
This review represents a summary of the development and application of a novel mixed-mode HPLC approach to the separation and analysis of peptides and proteins termed hydrophilic interaction/cation-exchange chromatography (HILIC/CEX). This approach combines the most advantageous aspects of two widely different separation mechanisms, i.e. a separation based on hydrophilicity/hydrophobicity differences between polypeptides overlaid on a separation based on net charge. Applications described include HILIC/CEX separations of cyclic peptides, alpha-helical peptides, random coil peptides and modified or deletion products of synthetic peptides. In addition, the excellent resolving ability of HILIC/CEX for modified histone proteins is described. This approach is shown to represent an excellent complement to RP chromatography (RPC), as well as being a potent analytical tool in its own right.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774265 | PMC |
| http://dx.doi.org/10.1002/jssc.200800243 | DOI Listing |
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