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Optimizing sample handling for urinary proteomics. | LitMetric

Optimizing sample handling for urinary proteomics.

J Proteome Res

Department of Urology, Urological Diseases Research Center, Harvard Medical School and Children's Hospital Boston, Boston, Massachusetts 02115, USA.

Published: September 2008

AI Article Synopsis

Article Abstract

Interrogation of the urinary proteome for clinically useful biomarkers of disease will require normalization of methods for protein extraction and sample handling. Variations in collection methods and other procedures may introduce significant discrepancies in qualitative and quantitative measurements. Here we demonstrate that the method of protein extraction, length of handling at room temperature, and repetitive freeze-thaw cycles do not seem to alter the urinary proteome at either the protein or peptide level in a manner that degrades information obtainable by mass spectrometry.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5451898PMC
http://dx.doi.org/10.1021/pr800301hDOI Listing

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