Based on the survey of the fishes in low salinity intertidal area of the Yangtze estuary from spring (May) 2006 to winter (February) 2007, the seasonal and semi-lunar changes of fish species and abundances were analyzed. A total of 53 fish species were collected, most of which belonged to Cyprinidae, followed by Gobiidae, and few of other families. The fish species and average abundance were higher in spring, summer, and autumn than in winter and lower in low tides than in high tides in four seasons. Shannon-Weaver (H') value had a greater fluctuation in a tidal cycle, and was appreciably lower in low tides than in high tides. The fish species and abundance were clustered into two groups, i. e., that of winter, and of the other seasons. As for the whole year, the abundance had the highest positive correlation with water temperature, then with tide height, and lowest with water salinity.
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