Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
The growth characteristics and the decontamination of heavy metals in analogous heavy metals wastewater by transgenic Synechococcus sp. PCC 7002 with mouse metallothionein-I gene were studied. The results show that transgenic Synechococcus sp. PCC 7002 not only has a higher tolerance to heavy metals, but also has a higher growth rate than wild strain. The concentration of Cd2+, Pb2+ and Hg2+ decreases with the progress of cultivation, and its maximum decreasing extent occurs at 1 - 3 day. After three days of cultivation, the absorption of Cd2+, Pb2+ and Hg2+ by transgenic Synechococcus sp. PCC 7002 is 10.75, 58.89 and 112.61 mg g(-1) of dried cells respectively, which is 3.16, 2.18 and 100.45 times higher than wild cells. The mono-factor and overall model developed fit the experiment data well.
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