We investigated the effects of tissue samples taken from rat brain on the reliability of three protein quantification kits: the Bradford assay, the 2-D Quant Kit, and the EZQ Protein Quantitation Kit. All three assays measured significantly smaller amounts of protein after extraction than the reference values before extraction. Only small effects were seen in homogenates, but very pronounced differences in membrane-enriched and highly lipophilic subcellular fractions. Researchers should evaluate which method of protein quantification is best qualified for their specific experimental design.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/pmic.200800236 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sensitive and accurate proteome profiling of embryogenesis using Real-Time Search and TMTproC quantification.
Mol Cell Proteomics
December 2024
Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, United States; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, United States; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States. Electronic address:
Multiplexed proteomics has become a powerful tool for investigating biological systems. Using balancer-peptide conjugates (e.g.
View Article and Find Full Text PDFTailored protein corona behavior in titanium dioxide nanosheet fluorescence biosensor for protein quantification assays.
J Colloid Interface Sci
December 2024
Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin 130012, China. Electronic address:
The spontaneous adsorption of proteins onto nanoparticles, known as the protein corona, provides a unique perspective for designing protein-sensing biosensors. This study proposes a tailored protein corona method mediated by Tween-20 and develops a reverse-capture approach for protein quantification assays. The protein-coated microplate captures titanium dioxide nanosheets (TiO-NS) in a phosphate buffer containing Tween-20 and generates fluorescence signals via the photocatalytic reduction of resazurin to resorufin, thereby indicating the amount of protein.
View Article and Find Full Text PDFSimultaneous determination of three β-Lactam/β-lactamase inhibitor combinations in critically ill patients by UPLC-MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci
December 2024
Department of Pharmacy, China-Japan Friendship Hospital, Beijing 100029, China. Electronic address:
β-Lactam/β-lactamase inhibitors (BL/BLIs) are widely used in critically ill patients. Recent research has shown the importance of therapeutic drug monitoring (TDM) of BLs, but few studies have highlighted the importance of detecting BLIs in critically ill patients. In our laboratory, we have developed and validated a simple and robust method for the determination of ceftazidime, cefoperazone, piperacillin, avibactam, sulbactam and tazobactam in human plasma by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
View Article and Find Full Text PDFModulation of IL-6 receptor/STAT3 downstream signaling in rheumatoid arthritis patients.
Exp Mol Pathol
December 2024
Rheumatology Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePre-J), University of Bari, Bari, Italy.
Interleukin-6 (IL-6) is a relevant cytokine in rheumatoid arthritis (RA) pathogenesis, potentially activating Janus kinases (JAK)-1, -2, and tyrosine kinase 2 (TYK2), and thus, three signal transducer and activator of transcription (STAT)-1, -3 or - 5 pathways. This pilot study aims to explore differences in phosphorylated (p)STAT3 levels among patients with RA, those not classified as RA (nRA), and healthy donors (HD), providing some clues on the relative contribution of each JAK protein to the downstream of the IL-6-induced STAT3 pathway. Clinical data and blood samples from 80 subjects (41 RA, 14 nRA, and 25 HD) were collected.
View Article and Find Full Text PDFDevelopment of marker-based quantification methods fo using DoE approach and anti-diabetic screening of selected phytoconstituents of the genus.
Nat Prod Res
December 2024
Department of Pharmaceutical Chemistry, KLE College of Pharmacy, KLE Academy of Higher Education and Research, JNMC Campus, Belagavi, Karnataka, India.
Diabetes mellitus is a rising global health issue, necessitating effective and affordable treatments. This study aimed to develop marker-based quantification methods for using a DoE approach and conduct anti-diabetic screening of its phytoconstituents. Methanolic extracts of the plant underwent fractionation, with the chloroform fraction used for simultaneous HPLC quantification of Plumbagin and Juglone.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!