[Concentration of IL-10, IL-13 and IFN-gamma cytokines in supernatants of microcultured CD4+ and NK cells in patients with non-allergic bronchial asthma].

Unlabelled: THE AIM OF THIS STUDY was the evaluation of T lymphocytes and NK function in patients with non-allergic asthma.

Material And Methods: Three groups: patients with non-allergic asthma (n = 30), patients with non-allergic asthma with concomitant recurrent infections (n = 24) and group of healthy adult volunteers (n = 20) were examined. CD4+ and NK cells were isolated from peripheral blood by magnetic separation method. Cells were cultured and level of cytokine IL-10, IL-13, IFN-gamma in medium were measured.

Results: In patients with non-allergic asthma with recurrent infections mean value of IFN-gamma in medium from IL-15 + IL-12 stimulated NK cells was significantly lower compared with control group (p < 0.01). Mean value of IL-10 and IL-13 in medium from Con A stimulated CD4+ cells in patients with non-allergic asthma with recurrent infections were significantly higher than in control group (p < 0.05).

Conclusions: Multitudes of cell and humoral factors interactions overlap dysfunction of immunological system in patients with non-allergic asthma. It causes higher susceptibility to infections factors especially viruses. Viruses give feedback for escalation of infection and worsening of clinical course of disease. Knowing mechanisms of inflammation intensification would let us specific prophylaxis use (specific antinflammatory or immunomodulatory drugs).