AI Article Synopsis
- Axonal outgrowth is crucial for brain development and relies on sialic acid from glycoproteins on cell membranes.
- The study found that nerve growth factor increases the expression of an enzyme called GNE, which is important for sialic acid production, in PC12 cells.
- Interestingly, while overexpressing GNE promotes neurite outgrowth, it does not enhance sialic acid biosynthesis, suggesting a new role for GNE in this process that isn't tied to its usual enzymatic function.
Article Abstract
Axonal outgrowth is a prerequisite for the development of the most complex organ, the brain. It depends partially on the attachment of sialic acid on glycans of (sialo)-glycoproteins expressed on the plasma membrane. In our study, we showed that nerve growth factor-induced neurite outgrowth of PC12-cells enhances the expression of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine-kinase (GNE), the key enzyme for the biosynthesis of sialic acid. Furthermore, we could show that overexpression of GNE induces neurite outgrowth in PC12 cells. The neurite-outgrowth promoting activity of overexpressed GNE, however, does not lead to an increased biosynthesis of sialic acid. These data suggest a novel role of GNE during neurite outgrowth, which is independent to its specific enzymatic activity.
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| http://dx.doi.org/10.1097/WNR.0b013e32830b368a | DOI Listing |
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