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In vivo comparison of hard tissue regeneration with ovine mesenchymal stem cells processed with either the FICOLL method or the BMAC method.
J Craniomaxillofac Surg
September 2015
Department of Oral- and Maxillofacial Surgery, University Hospital Tuebingen, Osianderstr. 2-8, 72076 Tuebingen, Germany; Department of Oral and Craniomaxillofacial Surgery, University Medical Center Freiburg, Freiburg, Germany.
Introduction: The aim of this work was to analyse the suitability of mesenchymal stem cell isolation by FICOLL density centrifugation and the closed bone marrow aspirate concentrate (BMAC) system for sinus augmentation with bovine bone mineral (BBM) in the sheep model.
Methods: 16 sheep underwent sinus augmentation with BBM and MSCs; they were divided between two groups with survival points of 8 and 16 weeks. For the FICOLL control arm three, and for the BMAC test arm, five augmentations were performed for each time point.
Comparative studies on surface phenotypes of Theileria lestoquardi and T. annulata schizont-infected cells.
Parasitol Res
September 2001
Department of Parasitology, SWEPAR, National Veterinary Institute and Swedish University of Agricultural Sciences, Uppsala.
Phenotypes of sheep cell lines infected with Theileria lestoquardi or T. annulata were studied by flow cytometric analysis, following immunolabelling with a panel of monoclonal antibodies reacting to leukocyte differentiation antigens. Cell surface phenotypes of Theileria-infected sheep cell lines derived ex vivo and in vitro were compared, both with each other and with cell lines from cattle undergoing acute T.
View Article and Find Full Text PDF[Differential diagnosis of classical swine fever and border disease: seroepidemiological investigation of a pestivirus infection on a mixed sheep and swine farm].
Dtsch Tierarztl Wochenschr
May 2001
Institut für Virologie, Tierärztliche Hochschule Hannover.
During recent years neutralizing antibodies against Border Disease Virus (BDV) were found repeatedly in German pig herds. Consequently there was a demand for a differential diagnostic system. A permanent sheep cell line and BDV reference strain Moredun were chosen and were applied in a could be used case study.
View Article and Find Full Text PDFThe in vitro construction of a tissue engineered bioprosthetic heart valve.
Eur J Cardiothorac Surg
March 1997
Department of Cardiac Surgery, Children's Hospital, Harvard Medical School, Boston MA 02115, USA.
Problem: Heart valve replacement with either a nonliving xenograft or a mechanical prosthesis is an effective therapy for valvular heart disease. Both of these approaches have limitations, including their inability to grow, repair, and remodel. In addition, a mechanical prosthesis requires long-term anticoagulation therapy.
View Article and Find Full Text PDFIn vitro IgM and IgM rheumatoid factor production in response to Staphylococcus aureus Cowan I: evidence for the role of Leu-2-positive T suppressor cells and radiosensitive T helper cells.
Clin Exp Immunol
August 1987
Division of Rheumatology and Clinical Immunology, Ottawa General Hospital, University of Ottawa, Ontario, Canada.
Staphylococcus aureus Cowan I (SAC) is a potent stimulus of B cell proliferation and differentiation, the latter being T cell-dependent. It has been suggested that immunoglobulin and IgM rheumatoid factor (RF) production in response to SAC involves radiosensitive T helper cells. We studied normal peripheral blood mononuclear cell (PBMC) cultures to assess the roles of radiosensitive T cells and Leu-2 positive suppressor cells in the cellular control of SAC-stimulated IgM and IgM RF responses.
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