AI Article Synopsis
- A new cell line called MSU-TnT4 (TnT4) was created from Trichoplusia ni embryos to test its capabilities in producing membrane proteins using baculovirus expression vectors.
- The TnT4 cells showed a longer doubling time of 21 hours but produced the human neurotensin receptor 1-GFP fusion protein at about twice the level compared to Sf21 cells.
- Overall, using this new cell line with recombinant baculoviruses offers a quick way to assess the effectiveness of various cell lines in producing membrane proteins through the baculovirus expression vector system (BEVS).
Article Abstract
A new cell line, MSU-TnT4 (TnT4), was established from Trichoplusia ni embryos for use with baculovirus expression vectors and evaluated for its potential for membrane protein production. To evaluate membrane protein synthesis, recombinant baculoviruses were constructed to express the human neurotensin receptor 1 as an enhanced green fluorescent protein (GFP) fusion. TnT4 cells had a doubling time of 21 h and expressed the membrane-GFP fusion protein at approximately twice the level as Sf21 cells from the p10 promoter, as evaluated by GFP intensity. Expression of secreted alkaline phosphatase (SEAP) was similar to that of Sf21 cells. Expression of membrane-GFP fusion proteins in recombinant baculoviruses provides a rapid method for evaluating the potential of new cell lines for the production of membrane proteins using a baculovirus expression vector system (BEVS).
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| http://dx.doi.org/10.1007/s11626-008-9095-z | DOI Listing |
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