It has previously been demonstrated that peripheral blood leucocytes of the nurse shark (Ginglymostoma cirrhatum) are capable of in vitro chemotaxis. In the present study we have identified the chemotactic cells as the shark granulocyte and monocyte-macrophage. Chemotaxis assays were set up in blind well chambers. Cells migrating into the filter in a chemotaxis assay were identified morphologically by transmission electron microscopy. Migration, in response to LPS-activated rat serum, was accompanied by change in cell shape and reorganization of the cytoskeleton. Ultrastructural changes included formation of lamellipodia with cell polarization, submembranous accumulation of microfilaments at points of plasma membrane contact with filter surfaces, densification of the membrane at these points of engagement, appearance of bundles of intermediate filaments extending into the protruded lamellipodia, and presence of oriented microtubules in the cytoplasma (cellular cortex). The phagocytic and chemotactic properties of the shark granulocyte and monocyte-macrophage points to their being structural and functional equivalents of the mammalian neutrophil and macrophage.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0040-8166(89)90020-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Hematology and Plasma Chemistry Reference Values in Nursehound Shark () Maintained Under Human Care.
Front Vet Sci
July 2022
Fundación Oceanogràfic de la Comunitat Valenciana, Valencia, Spain.
Studies determining baseline hematological reference intervals (RI) in elasmobranchs are very limited. In this study, blood samples were collected from 94 clinically healthy Nursehound Shark () maintained under human care. Median (RI) in major leukocyte types were similar to other Carcharhinid sharks as lymphocytes were the predominant leukocyte with 38.
View Article and Find Full Text PDFCharacterization of the turbot Scophthalmus maximus (L.) myeloperoxidase. An insight into the evolution of vertebrate peroxidases.
Dev Comp Immunol
May 2021
Department of Fundamental Biology, Institute of Aquaculture, Campus Vida, University of Santiago de Compostela, E-15782, Santiago de Compostela, Spain. Electronic address:
We have completed the characterization of the turbot (Scophthalmus maximus) myeloperoxidase (mpx) gene and protein, which we partially described in a previous study. The turbot mpx gene has 15 exons that encode a protein of 767 aa, with a signal peptide, propeptide and light and heavy chains, and also with haem cavities, a Ca-binding motif and several N- and O-glycosylation sites. The mature protein forms homodimers of about 150 kDa and is very abundant in turbot neutrophils.
View Article and Find Full Text PDFBlood plasma levels of heavy metals and trace elements in white sharks (Carcharodon carcharias) and potential health consequences.
Mar Pollut Bull
May 2019
Department of Marine Ecosystems and Society, Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, Miami, FL 33149, USA; Leonard and Jayne Abess Center for Ecosystem Science and Policy, University of Miami, Coral Gables, FL 33146, USA.
Heavy metals may adversely affect health in marine organisms. As top predators, sharks may be especially vulnerable to exposure over long lifespans. Here we evaluate plasma levels of 14 heavy metals and 12 trace elements in white sharks, Carcharodon carcharias, in South Africa to determine whether they are related to sex, body size, and/or body condition and other health parameters.
View Article and Find Full Text PDFSeasonal fluctuation in environmental parameters can influence immune responses of vertebrates and consequently influence their health and disease resistance. Although seasonality of immune function is well documented in a broad range of vertebrate taxa, this information remains virtually unexplored in cartilaginous fish. Here we examine seasonal variation in immune and general-health parameters of free-living adult broadnose sevengill sharks, Notorynchus cepedianus, along an annual cycle.
View Article and Find Full Text PDFLocally extensive meningoencephalitis caused by Miamiensis avidus (syn. Philasterides dicentrarchi) in a zebra shark.
Dis Aquat Organ
October 2017
Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, Taipei 10617, Taiwan, ROC.
Scuticociliatosis, caused by ciliated protozoa in the subclass Scuticociliatia of the phylum Ciliophora, can cause fatal disease in teleost fish species. However, information on scuticociliatosis in elasmobranchs is still scarce. In this report, we describe a case of locally extensive meningoencephalitis caused by Miamiensis avidus (syn.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!