Objective: To determine the total content of astragaloside IV in Radix Astragali.
Methods: The measurement conditions were used as follows: Irregular C18 (4.6 mm x 250 mm, 5 microm) column; mobile phase: acetonitrile-water (32:68); flow rate: 1.0 ml/min; detector: ELSD 2000; the temperature of drift tube: 100 degrees C; gas flow: 2.7 L/min.
Results: Eight batches of Radix Astragali from different sources were determined. The stability, precision and reproducibility of the method were studied, RSD <3%.
Conclusion: There is great difference between the content of astragaloside IV in Radix Astragali by different processing methods.
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