Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
The resolving power of multiple-locus variable-number tandem repeat analysis (MLVA) was undertaken on 78 phenotypically closely related isolates of Salmonella enterica serovar Typhimurium that were previously analysed with multiple amplification of phage locus typing (MAPLT) and pulsed-field gel electrophoresis (PFGE). Isolates were tested with 10 primer sets targeting tandem repeat loci in the S. Typhimurium genome. A new primer set targeting the SB21(ST64B) locus was also assessed for MAPLT analysis. Both methods produced similar levels of discrimination between 41 non-DT 126 isolates while MLVA proved superior in separating the DT 126 isolates. Results showed that two loci, STTR5 and STTR6, provided the most variation in tandem repeat numbers between the 78 S. Typhimurium isolates with 11 alleles detected for each locus. Some isolates did not produce amplified product with PCR for various loci, providing another level for discrimination. The remaining MLVA loci provided less allelic variation, with 4 loci failing to provide any discrimination. There were a number of isolates that were shown to be unique by one method but were clustered by the other method. Combining the most variable regions of both MAPLT and MLVA into one assay may provide significant resolution of isolates with the minimal number of primers utilised.
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Source |
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http://dx.doi.org/10.1016/j.ijmm.2008.05.009 | DOI Listing |
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