AI Article Synopsis
- Rift Valley fever virus (RVFV) is a mosquito-borne virus that causes diseases in humans and animals, primarily found in sub-Saharan Africa and introduced to the Arabian Peninsula in 2000.
- The researchers developed a reverse genetics method to recover infectious RVFV using cellular RNA polymerase I to synthesize viral transcripts, comparing its effectiveness with the T7 RNA polymerase system.
- Both systems were found to be equally efficient, producing high viral titers, and the RNA polymerase I method was used to rescue various RVFV strains, including attenuated and virulent variants, as well as chimeric and reporter protein-expressing viruses.
Article Abstract
Rift Valley fever virus (RVFV, Bunyaviridae, Phlebovirus) is a mosquito-transmitted arbovirus that causes human and animal diseases in sub-Saharan Africa and was introduced into the Arabian Peninsula in 2000. Here, we describe a method of reverse genetics to recover infectious RVFV from transfected plasmids based on the use of the cellular RNA polymerase I promoter to synthesize viral transcripts. We compared its efficiency with a system using T7 RNA polymerase and found that both are equally efficient for the rescue of RVFV generating titers of approx. 10(7) to 10(8) pfu/ml. We used the RNA polymerase I-based system to rescue both attenuated MP12 and virulent ZH548 strains as well as chimeric MP12-ZH548 viruses, and in addition RVFV expressing reporter proteins.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577904 | PMC |
| http://dx.doi.org/10.1016/j.virol.2008.05.033 | DOI Listing |
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