With miniaturized tube lenses and a micro-electro-mechanical system (MEMS) mirror, we constructed a miniaturized multiphoton microscope system. Through a two-dimensional asynchronous scanning of the MEMS mirror, 24Hz frame rate can be realized. With a high numerical aperture objective, sub-micron resolution can also be achieved at the same time.
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http://dx.doi.org/10.1364/oe.16.010501 | DOI Listing |
bioRxiv
October 2024
Department of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Multiphoton microscopy combined with optogenetic photostimulation is a powerful technique in neuroscience enabling precise control of cellular activity to determine the neural basis of behavior in a live animal. Two-photon patterned photostimulation has taken this further by allowing interrogation at the individual neuron level. However, it remains a challenge to implement imaging of neural activity with spatially patterned two-photon photostimulation in a freely moving animal.
View Article and Find Full Text PDFJ Comp Neurol
April 2024
Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, USA.
Comprehensive understanding of interconnected networks within the brain requires access to high resolution information within large field of views and over time. Currently, methods that enable mapping structural changes of the entire brain in vivo are extremely limited. Third harmonic generation (THG) can resolve myelinated structures, blood vessels, and cell bodies throughout the brain without the need for any exogenous labeling.
View Article and Find Full Text PDFNeurophotonics
September 2024
Sorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France.
Optogenetics opened the door to a new era of neuroscience. New optical developments are under way to enable high-resolution neuronal activity imaging and selective photostimulation of neuronal ensembles in freely moving animals. These advancements could allow researchers to interrogate, with cellular precision, functionally relevant neuronal circuits in the framework of naturalistic brain activity.
View Article and Find Full Text PDFACS Omega
September 2023
National Electronics and Computer Technology Center, 112 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
We demonstrate quantum random number generation based on a photon-number detection scheme with the use of a silicon photomultiplier. We implement a time integral with detector response signals for resolving photon numbers, which are subsequently digitized into a stream of 4-bit sequences with a generation rate of 13.6 Mbit/s.
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